Please use this identifier to cite or link to this item:https://hdl.handle.net/20.500.12259/60670
Type of publication: Konferencijų tezės nerecenzuojamuose leidiniuose / Conference theses in non-peer-reviewed publications (T2)
Field of Science: Biofizika / Biophysics (N011)
Author(s): Navickaitė, Diana;Maciulevičius, Martynas;Chopra, Sonam;Šatkauskas, Saulius
Title: Calcium delivery via electroporation significantly enhances cell death in vitro
Is part of: Bioelectrics 2018: 15th international symposium, Prague, Czech Republic, September 23rd - 26th, 2018: book of abstracts. Prague : Institute of plasma physics of the CAS, 2018
Extent: p. 1-1
Date: 2018
Note: http://www.bioelectrics2018.cz
Keywords: Electroporation;Chemotherapy;Cell death;Chinese hamster ovary cells
ISBN: 9788087026106
Abstract: The intracellular/intratumoral delivery of chemotherapeutic drugs using electroporation has been widely investigated as a promising modality for cancer treatment. Recently it has been discovered that calcium ion delivery via electroporation can also kill cancerous cells without posing harm to neighbour healthy tissue. The increase of intracellular calcium concentration can significantly induce cell death and tumour necrosis, thus calcium electroporation is considered to be an effective alternative to electrochemotherapy using anticancer drugs such as bleomycin or cisplatin [1]. Cell death is induced by the sudden change in calcium concentration inside the cell which is related to ATP depletion, lipase and protease activity, the production of reactive oxygen species, non-specific pore opening, etc [2]. Despite the plethora of investigations performed, the true mechanism behind calcium electroporation remains unclear. In addition, electroporation parameters for effective calcium delivery to cells have to be determined. In this research we have analysed Chinese hamster ovary cell viability and metabolic activity using two different assays: cell clonogenic and MTT. The cells were exposed to different CaCl2 concentration (0, 0.1, 0.2, 0.5, 1, 2, 3, 4, 5 mM) media and electroporation pulses (1; 2; 4 HV). After the application of high voltage electric pulses (1200 V/cm, 100 μs), cell viability as well as metabolic activity (after 0.5, 12, 24 h) decrease with increasing calcium concentration. As our results have shown it is possible to obtain 20 - 100 % cell death depending on the electroporation parameters and calcium concentrations used. In addition to this the increase in cell viability after electroporation application was observed at lower calcium concentrations (in the range of 0,1 – 0,5 mM), which shows that low calcium levels can stimulate cell survival. [...]
Internet: https://hdl.handle.net/20.500.12259/60670
Affiliation(s): Biologijos katedra
Gamtos mokslų fakultetas
Vytauto Didžiojo universitetas
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications

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