Vytautas Magnus University Research Management System (VDU CRIS)
 
Use this url to cite publication: https://hdl.handle.net/20.500.12259/60590

Physicochemical properties and in vitro cytotoxicity studies of chitosan as a potential agent for cancer treatment

Type of publication
Konferencijų tezės nerecenzuojamame leidinyje / Conference theses in non-peer-reviewed publication (T2)
Author(s)
AuthorAffiliation
Oberemko, Alona
Gamtos mokslų fakultetas / Faculty of Natural Sciences
LT
Salaberria, Asier M.
University of the Basque Country
Title
Physicochemical properties and in vitro cytotoxicity studies of chitosan as a potential agent for cancer treatment
[en]
Is part of
New aspects on chemistry and application of chitin and its derivatives, 24th conference of Polish Chitin Society, Tyniec, September 19-21st 2018: abstracts book. Tyniec : Polish Chitin Society, 2018
Date Issued
Date
2018
Publisher
Tyniec : Polish Chitin Society, 2018
Extent
p. 53-54
URI
URI
http://www.ptchit.lodz.pl/file-PTChit_(5csjmejo12rwob0m).pdf
https://hdl.handle.net/20.500.12259/60590
Field of Science
Keywords (en)
Abstract (en)

The mechanisms responsible for the cytotoxic effects of chitosan on cancer cells are still unclear. The goal of this study was to evaluate the physicochemical and cytotoxic properties of chitosan in vitro on mouse hepatoma MH-22A (tumor cells) and Chinese hamster ovary (CHO, noncancerous) cells cultivated on films with different chitosan concentrations and cultivated on Petri dishes after preincubation with chitosan solutions. The chitins and chitosans of different origin (crustacean, insect, mushroom) were characterized by attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR), elemental analyses (EA), nuclear magnetic resonance spectroscopy (NMR), thermogravimetric analyses (TGA) and X-ray diffraction (XRD). To measure plating efficiency (cloning efficiency), cells were plated at low densities on chitosan films or after preincubation with chitosan solutions cells were plated on Petri dishes and allowed to grow. To see the colonies clearly, they were stained with crystal violet. To reveal the rupture of cell membrane (morphologic expression of necrosis) cells were stained with Trypan Blue solution after 40 and 100 min of chitosan treatment.[...]

Type of document
type::text::conference output::conference proceedings::conference paper
Language
Anglų / English (en)
Coverage Spatial
Lenkija / Poland (PL)
Other Identifier(s)
VDU02-000023598