Please use this identifier to cite or link to this item:https://hdl.handle.net/20.500.12259/58395
Type of publication: Straipsnis konferencijos medžiagoje Clarivate Analytics Web of Science ar/ir Scopus / Article in Clarivate Analytics Web of Science or Scopus DB conference proceedings (P1a)
Field of Science: Biofizika / Biophysics (N011)
Author(s): Tamošiūnas, Mindaugas;Jakovels, D;Ļihačovs, A;Kilikevičius, Audrius;Baltušnikas, Juozas;Kadikis, R;Šatkauskas, Saulius
Title: Application of fluorescence spectroscopy and multispectral imaging for non-invasive estimation of GFP transfection efficiency
Is part of: AOMD-8 : eighth international conference on advanced optical materials and devices, 25-27 August 2014, Riga, Latvia [elektroninis išteklius]. Bellingham : SPIE Press, 2014
Extent: p. 1-10
Date: 2014
Series/Report no.: (Procedings of SPIE, Vol. 9421 0277-786X)
Keywords: Fluorescence;Spectroscopy;Multispectral imaging;DNA transfection
ISBN: 9781628415209
Abstract: Electroporation and ultrasound induced sonoporation has been showed to induce plasmid DNA transfection to the mice tibialis cranialis muscle. It offers new prospects for gene therapy and cancer treatment. However, numerous experimental data are still needed to deliver the plausible explanation of the mechanisms governing DNA electro- or sono-transfection, as well as to provide the updates on transfection protocols for transfection efficiency increase. In this study we aimed to apply non-invasive optical diagnostic methods for the real time evaluation of GFP transfection levels at the reduced costs for experimental apparatus and animal consumption. Our experimental set-up allowed monitoring of GFP levels in live mice tibialis cranialis muscle and provided the parameters for DNA transfection efficiency determination
Internet: http://spie.org/Publications/Proceedings/Paper/10.1117/12.2083895
http://spie.org/Publications/Proceedings/Paper/10.1117/12.2083895
Affiliation(s): Biologijos katedra
Gamtos mokslų fakultetas
Lietuvos sporto universitetas
Vytauto Didžiojo universitetas
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications

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