Please use this identifier to cite or link to this item:https://hdl.handle.net/20.500.12259/58393
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dc.contributor.authorTamošiūnas, Mindaugas-
dc.contributor.authorKadikis, Roberts-
dc.contributor.authorSaknite, Inga-
dc.contributor.authorBaltušnikas, Juozas-
dc.contributor.authorKilikevičius, Audrius-
dc.contributor.authorLihachev, Alexey-
dc.contributor.authorPetrovska, Ramona-
dc.contributor.authorJakovels, Dainis-
dc.contributor.authorŠatkauskas, Saulius-
dc.coverage.spatialUS-
dc.date.accessioned2018-10-07T01:42:47Z-
dc.date.available2018-10-07T01:42:47Z-
dc.date.issued2016-
dc.identifier.issn10833668-
dc.identifier.otherVDU02-000019290-
dc.identifier.urihttps://doi.org/10.1117/1.JBO.21.4.045003-
dc.descriptionE-ISSN: 1560-2281. Impact Factor: 2.859-
dc.description.abstractWe highlight the options available for noninvasive optical diagnostics of reporter gene expression in mouse tibialis cranialis muscle. An in vivo multispectral imaging technique combined with fluorescence spectroscopy point measurements has been used for the transcutaneous detection of enhanced green fluorescent protein (EGFP) expression, providing information on location and duration of EGFP expression and allowing quantification of EGFP expression levels. For EGFP coding plasmid (pEGFP-Nuc Vector, 10  μg/50  ml10  μg/50  ml) transfection, we used electroporation or ultrasound enhanced microbubble cavitation [sonoporation (SP)]. The transcutaneous EGFP fluorescence in live mice was monitored over a period of one year using the described parameters: area of EGFP positive fibers, integral intensity, and mean intensity of EGFP fluorescence. The most efficient transfection of EGFP coding plasmid was achieved, when one high voltage and four low voltage electric pulses were applied. This protocol resulted in the highest short-term and long-term EGFP expression. Other electric pulse protocols as well as SP resulted in lower fluorescence intensities of EGFP in the transfected area. We conclude that noninvasive multispectral imaging technique combined with fluorescence spectroscopy point measurements is a suitable method to estimate the dynamics and efficiency of reporter gene transfection in vivoen
dc.description.sponsorshipBiochemijos katedra-
dc.description.sponsorshipBiologijos katedra-
dc.description.sponsorshipLietuvos sporto universitetas-
dc.description.sponsorshipVytauto Didžiojo universitetas-
dc.format.extentp. 1-12-
dc.language.isoen-
dc.relation.ispartofJournal of biomedical optics. Bellingham, WA : SPIE, 2016, Vol. 21, iss. 4-
dc.relation.isreferencedbyScience Citation Index Expanded (Web of Science)-
dc.relation.isreferencedbyMEDLINE-
dc.relation.isreferencedbyInspec-
dc.relation.isreferencedbyAcademic Search Premier (EBSCO)-
dc.relation.isreferencedbyScopus-
dc.subjectSonoporationen
dc.subjectElectroporationen
dc.subjectGreen fluorescent proteinsen
dc.subjectMultispectral imagingen
dc.subjectFluorescence spectroscopyen
dc.subject.otherBiologija / Biology (N010)-
dc.titleNoninvasive optical diagnostics of enhanced green fluorescent protein expression in skeletal muscle for comparison of electroporation and sonoporation efficienciesen
dc.typeStraipsnis Clarivate Analytics Web of Science ar/ir Scopus / Article in Clarivate Analytics Web of Science or / and Scopus (S1)-
dc.identifier.doihttps://doi.org/10.1117/1.JBO.21.4.045003-
dc.identifier.isiWOS:000375929300005-
dcterms.bibliographicCitation48-
dc.date.updated2020-01-30T14:39Z-
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local.typeS-
item.grantfulltextopen-
item.fulltextWith Fulltext-
crisitem.author.deptBiochemijos katedra-
crisitem.author.deptPolitikos mokslų ir diplomatijos fakultetas-
crisitem.author.deptPolitikos mokslų ir diplomatijos fakultetas-
crisitem.author.deptBiologijos katedra-
crisitem.author.deptPolitikos mokslų ir diplomatijos fakultetas-
crisitem.author.deptPolitikos mokslų ir diplomatijos fakultetas-
crisitem.author.deptPolitikos mokslų ir diplomatijos fakultetas-
crisitem.author.deptPolitikos mokslų ir diplomatijos fakultetas-
crisitem.author.deptBiologijos katedra-
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications
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