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Type of publication: conference paper
Type of publication (PDB): Konferencijų tezės nerecenzuojamuose leidiniuose / Conference theses in non-peer-reviewed publications (T2)
Field of Science: Biologija / Biology (N010)
Author(s): Tamoliūnaitė, Dovilė;Radzijevskaja, Jana;Paulauskas, Algimantas;Sabūnas, Vytautas;Karvelienė, Birutė;Zamokas, Gintaras
Title: Identification of Babesia canis genotypes in dogs from Lithuania
Is part of: COINS 2018: 13th international conference of life sciences, 28 February – 2 March 2018: book of abstracts. Vilnius: Vilniaus universitetas, Gyvybės mokslų centras, 2018
Extent: p. 77-78
Date: 2018
Note: This research is funded by the European Social Fund under the No. 09.3.3-LMT-K-712-03-0081 “Development of Competences of Scientists, other Researchers and Students through Practical Research Activities” measure
Keywords: Babesia canis;Tick-borne disease;Dogs
Abstract: Canine babesiosis is a widespread tick-borne disease caused by haematozoan parasites of the genus Babesia. The vast majority of clinical babesiosis cases in dogs in Europe is caused by Babesia canis. Canine babesiosis has become quite frequent in Lithuania during the past decade. Babesiosis caused by B. canis may range from mild to severe disease in dogs. Such difference in the virulence of B. canis strains is associated with genetic heterogeneity among B. canis strains. We aimed to investigate the genetic diversity of B. canis strains isolated from naturally infected dogs in Lithuania using PCR-RFLP assay and sequence analysis based on 18S rRNR gene. In total 138 blood samples from dogs suspected of babesiosis were collected in Lithuania during 2016-2017. DNA was isolated from EDTA-anticoagulated whole blood. A partial region of the 18S rRNA gene of B. canis was amplified through PCR. PCR products were submitted to digestion with HincII restriction enzyme and evaluated by agarose gel electrophoresis. PCR products of 18S rRNA gene were sequenced and then analyzed using the Mega software.[...]
Affiliation(s): Biologijos katedra
Gamtos mokslų fakultetas
Lietuvos sveikatos mokslų universitetas. Veterinarijos akademija
Vytauto Didžiojo universitetas
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications

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