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Type of publication: Straipsnis Clarivate Analytics Web of Science ar/ir Scopus / Article in Clarivate Analytics Web of Science or / and Scopus (S1)
Field of Science: Biofizika / Biophysics (N011);Elektros ir elektronikos inžinerija / Electrical and electronic engineering (T001)
Author(s): Novickij, Vitalij;Ruzgys, Paulius;Grainys, Audrius;Šatkauskas, Saulius
Title: High frequency electroporation efficiency is under control of membrane capacitive charging and voltage potential relaxation
Is part of: Bioelectrochemistry. Amsterdam : Elsevier B.V., 2018, Vol. 119
Extent: p. 92-97
Date: 2018
Keywords: Membrane permeabilization;Propidium iodide;Transmembrane potential;Cell polarization;MHz
Abstract: The study presents the proof of concept for a possibility to achieve a better electroporation in theMHz pulse repetition frequency (PRF) region compared to the conventional low frequency protocols. The 200 ns × 10 pulses bursts of 10–14 kV/cm have been used to permeabilize Chinese hamster ovary (CHO) cells in a wide range (1 Hz–1 MHz) of PRF. The permeabilization efficiency was evaluated using fluorescent dye assay (propidium iodide) and flow cytometry. It was determined that a threshold PRF exists when the relaxation of the cell transmembrane potential is longer than the delay between the consequent pulses, which results in accumulation of the charge on the membrane. For the CHO cells and 0.1 S/melectroporationmedium, this phenomenon is detectable in the 0.5–1MHz range. Itwas shown that the PRF is an important parameter that could be used for flexible control of electroporation efficiency in the high frequency range
Affiliation(s): Aplinkos tyrimų centras
Biologijos katedra
Gamtos mokslų fakultetas
Vilniaus Gedimino technikos universitetas
Vilnius Gediminas Technical University, Lithuania
Vytauto Didžiojo universitetas
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications

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