Please use this identifier to cite or link to this item:https://hdl.handle.net/20.500.12259/57406
Type of publication: research article
Type of publication (PDB): Straipsnis kitose duomenų bazėse / Article in other databases (S4)
Field of Science: Biofizika / Biophysics (N011)
Author(s): Meyer, Lionel A.T;Kaselis, Andrius;Šatkauskas, Saulius;Bagnard, Dominique
Title: Analysis of semaphorin-induced growth cone collapse and axon growth inhibition
Is part of: Semaphorin signaling: methods in molecular biology / edited by Jonathan R. Terman. New York : Humana Press, 2017
Extent: p. 171-183
Date: 2017
Note: Online ISBN 978-1-4939-6448-2. DOI 10.1007/978-1-4939-6448-2. Series: Methods in Molecular Biology; Volume 1493; ISSN 1064-3745
Keywords: Cytoskeleton;Dorsal root ganglia;Gradients;Plexin;Sensory neurons
ISBN: 9781493964468
Abstract: The axonal growth cone is a specialized structure enabling axon extension and proper guidance to its target by sensing the extracellular environment. A growth cone collapse assay is a popular approach designed to characterize the inhibitory effect of secreted guidance cues in vitro. However, the actin cytoskeleton of the growth cone is very sensitive to various factors like physical impact, temperature, and acidity of environment that may also induce responses resembling those of guidance signals. Herein, we provide an easy and reproducible method to analyze growth cone sensitivity to the prototypic guidance molecule family class 3 semaphorin. This protocol is intended to present a systematic approach that is easy to apply to any soluble factors with a potential to impact axon elongation
Internet: https://hdl.handle.net/20.500.12259/57406
Affiliation(s): Biologijos katedra
Vytauto Didžiojo universitetas
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications

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