Phytochemical analysis diploid and polyploid forms of ribes using spectrophotometric, liquid and gas chromatographic methods

Abstract

Polyploid plants can be created using biotechnological modification methods. New plant can differ from the primary form: anatomical features of the plant, different quality and quantity of secondary metabolites and different size fruits. Blackcurrants are cultivated as industrial applications and mainly used of berries. In folk medicine the leaves and buds of blackcurrant are used for tea. The tea can be used for treatment of rheumatism, arthritis and gastric disorders [1]. Blackcurrants diploid and polyploid forms that were grown in Lithuanian institute of horticulture were used for phytochemical research [2]. Volatile aroma compounds of diploid and polyploid forms of Ribes buds and stalks were analysed by gas chromotography. The qualitative analysis of total content of phenolic compounds was completed using spectrofotometric methods with Folin – Ciocalteu reagent. Flavonoid content was determined using reaction with aluminum chloride. Radical scavenging activity was measured using DPPH radical. With liquid chromatography were identified phenol compounds and their contents of blackcurrants methanol extract. Liquid chromatography was used with reversed – phase column with reaction detector