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Type of publication: conference paper
Type of publication (PDB): Tezės kituose recenzuojamuose leidiniuose / Theses in other peer-reviewed publications (T1e)
Field of Science: Biochemija / Biochemistry (N004)
Author(s): Dūda, Gediminas;Maruška, Audrius;Drevinskas, Tomas
Title: Optimization of capillary electrophoresis-UV absorption detection method for analysis of thymol and carvacrol in essential oil of Thymus serpyllum L
Is part of: The vital nature sign [electronic resource] :10th international scientific conference, May 19-20, 2016 Vilnius, Lithuania : abstract book. Kaunas : Vytautas Magnus university, 2016, [no. 10]
Extent: p. 20-20
Date: 2016
Keywords: Capillary electrophoresis;Wild Thyme;Tymol;Carvarcol;UV absorption detection;Esential oil
Abstract: Wild Thyme (Thymus serpyllum L.) is a medicinal plant, native to most of Europe. In Lithuania wild thyme usually grows in loam soil and in dry meadows. Wild thyme is often used in herbal medicine and as ingriedient in cooking. Biologically active compounds are most often found in essential oil. About forty-five different chemical compounds where found in essential oil Thymol and carvacrol are two main compounds in essential oil. Both thymol and carvacrol have strong antimicrobial and antifungal properties. Plant material was collected in Utena county. Essential oil extractions were conducted as following: 0.5 grams of dried plant material was extracted for 24 hours in 75% and 100% methanol. Capillary zone electrophoresis method with UV absorption detector was optimized and used for determination of thymol and carvacrol in the extract of Thymus serpyllum L. Optimized conditions were following: 25mM pH=9,35 sodium tetraborate soliution was used as a background electrolyte. 30mM sodium hydroxide solution was used washing the capillary. Applied voltage was 25kV, total capillary length was 60 cm, capillary length to detector was 50 cm, separation temperature was 23°C
Affiliation(s): Gamtos mokslų fakultetas
Instrumentinės analizės APC
Vytauto Didžiojo universitetas
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications

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