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Type of publication: conference paper
Type of publication (PDB): Konferencijų tezės nerecenzuojamuose leidiniuose / Conference theses in non-peer-reviewed publications (T2)
Field of Science: Biologija / Biology (N010)
Author(s): Drevinskas, Tomas;Mickienė, Rūta;Tiso, Nicola;Maruška, Audrius;Šalomskas, Algirdas;Lelešius, Raimundas;Karpovaitė, Agneta;Ragažinskienė, Ona;Kubilienė, Loreta
Title: Determination of amino acids using capillary electrophoresis - contactless conductivity detection in antiviral medicinal plants
Is part of: ExTech 2016, ISSS 2016: 18th international symposium on advances in extraction technologies; 22nd international symposium on separation sciences, 3-6 July, 2016, Torun, Poland : abstract book / editors B. Buszewski, J. Kowalska. Torun: Nicolaus Copernicus University, 2016
Extent: p. 124-124
Date: 2016
Abstract: Medicinal plants are valued due to accumulated polyphenolic compounds, however amines and amino acids are rarely investigated. Compounds containing amino group are considered as biologically active: some of them act as the mediators, other can be the cause of poisoning. In this work capillary electrophoresis coupled with capacitance-to-digital based miniaturized contactless conductivity detector was used for determination of amino acids and other amine group containing substances. Sixteen different medicinal plants were selected for screening. Analytes were separated in acidic (acetic acid solution) background electrolyte ensuring that all amine containing compounds are dissociated and charged positively. After the injection and application of electric field cations start migrating from anode to cathode and are detected by contactless conductivity detector in negative detection mode. Separated compounds were classified as having high, medium and low electrophoretic mobility. Different plant extracts indicated different peak profile. Several amino acids were identified and determined in all plant extracts. The compounds determined and conditions used for this research work will be presented during the conference
Affiliation(s): Botanikos sodas
Instrumentinės analizės APC
Lietuvos sveikatos mokslų universitetas. Medicinos akademija
Lietuvos sveikatos mokslų universitetas. Veterinarijos akademija
Lietuvos sveikatos mokslų universitetas. Veterinarijos akademija. Mikrobiologijos ir virusologijos institutas
Vytauto Didžiojo universitetas
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications

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