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Type of publication: research article
Type of publication (PDB): Straipsnis Clarivate Analytics Web of Science / Article in Clarivate Analytics Web of Science (S1)
Field of Science: Chemijos inžinerija / Chemical engineering (T005)
Author(s): Drevinskas, Tomas;Mickienė, Rūta;Maruška, Audrius;Stankevičius, Mantas;Tiso, Nicola;Mikašauskaitė, Jurgita;Ragažinskienė, Ona;Levišauskas, Donatas;Bartkuvienė, Violeta;Snieškienė, Vilija;Stankevičienė, Antanina;Polcaro, Chiara;Galli, Emanuela;Donati, Enrica;Tekorius, Tomas;Kornyšova, Olga;Kaškonienė, Vilma
Title: Downscaling the in vitro test of fungal bioremediation of polycyclic aromatic hydrocarbons : methodological approach
Is part of: Analytical and bioanalytical chemistry. Berlin/Heidelberg : Springer, 2016, Vol. 408, iss. 4
Extent: p. 1043-1053
Date: 2016
Note: ISSN 1618-2650 (Online)
Keywords: Miniatūrizacija;Bioremediacija;Policikliniai aromatiniai angliavandeniai;Ultraefektyvioji skysčių chromatografija;Miniaturization;Bioremediation;PAH;UPLC;White rot fungi
Abstract: The miniaturization and optimization of a white rot fungal bioremediation experiment is described in this paper. The optimized procedure allows determination of the degradation kinetics of anthracene. The miniaturized procedure requires only 2.5 ml of culture medium. The experiment is more precise, robust, and better controlled comparing it to classical tests in flasks. Using this technique, different parts, i.e., the culture medium, the fungi, and the cotton seal, can be analyzed. A simple sample preparation speeds up the analytical process. Experiments performed show degradation of anthracene up to approximately 60 % by Irpex lacteus and up to approximately 40 % by Pleurotus ostreatus in 25 days. Bioremediation of anthracene by the consortium of I. lacteus and P. ostreatus shows the biodegradation of anthracene up to approximately 56 % in 23 days. At the end of the experiment, the surface tension of culture medium decreased comparing it to the blank, indicating generation of surfactant compounds
Affiliation(s): Biochemijos katedra
Botanikos sodas
Instrumentinės analizės APC
Kauno technologijos universitetas
Vytauto Didžiojo universitetas
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications

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