Please use this identifier to cite or link to this item:https://hdl.handle.net/20.500.12259/56948
Type of publication: Straipsnis Clarivate Analytics Web of Science ar/ir Scopus / Article in Clarivate Analytics Web of Science or / and Scopus (S1)
Field of Science: Farmacija / Pharmacy (M003);Biochemija / Biochemistry (N004);Chemijos inžinerija / Chemical engineering (T005)
Author(s): Ugenskienė, Rasa;Juozaitytė, Elona;Ramanauskienė, Kristina;Briedis, Vitalis;Maruška, Audrius;Raulinaitytė, Danguolė;Kaškonienė, Vilma;Drevinskas, Tomas;Stelmakienė, Ada;Akuneca, Ieva;Makaravičius, Tomas;Tiso, Nicola;Bartkuvienė, Violeta;Kornyšova, Olga;Ragažinskienė, Ona
Title: Analysis of antiproliferative effect of Chamerion angustifolium water extract and its fractions on several breast cancer cell lines / Audrius Maruška, Rasa Ugenskienė, Danguolė Raulinaitytė, Elona Juozaitytė, Vilma Kaškonienė, Tomas Drevinskas, Ada Stelmakienė, Ieva Akuneca, Tomas Makaravičius, Nicola Tiso, Violeta Bartkuvienė, Olga Kornyšova, Ona Ragažinskienė, Kristina Ramanauskienė, Vitalis Briedis
Is part of: Advances in medical sciences. Amsterdam : Elsevier, 2017, vol. 62, no. 1
Extent: p. 158-164
Date: 2017
Note: Original research article ; authors (15) ; the Study was financially supported by the Research Council of Lithuania (grant no. MIP 084/2012 ONKOFITAS)
Keywords: Breast neoplasms;drug therapy;Breast neoplasms;cytology;MCF7 cells;drug effects;Antineoplastic agents, phytogenic;pharmacology;Onagraceae;chemistry;Plant extracts;pharmacology
Abstract: PURPOSE: To evaluate the antiproliferative effect of the aerial part of Chamerion angustifolium (L.) Holub. (Onagraceae) extract and its fractions in vitro. This is the first study on the anti-proliferative effect of C. angustifolium on 3 distinct breast cancer cell lines. MATERIAL/METHODS: Breast cancer cell lines MCF7, MDA-MB-468 and MDA-MB-231 were exposed to different concentrations of the water extract of C. angustifolium, where DPPH radical scavenging activity was 0.018-0.443mg/ml, expressed in rutin equivalents. Cell growth was analyzed after 24, 48 and 72h of incubation. Solid-phase extraction was applied for the fractionation of C. angustifolium water extract and MDA-MB-468 cell line growth was tested using different fractions. RESULTS: The concentrations corresponding to radical scavenging activity of 0.117 and 0.266mg/ml caused MCF7 cells growth inhibition, while in the samples exposed to the highest concentration (0.355 and 0.443mg/ml) no proliferation was register, suggesting cell death. MDA-MB-468 cell analysis showed similar responses. MDA-MB-231 demonstrated cell growth inhibition following the exposure to all analyzed high extract doses (0.117-0.443mg/ml). MDA-MB-468 cells were selected to evaluate the effect of fractions. In the samples exposed to the fraction containing the highest amount (91%) of oenothein B, at the concentration of 0.117mg/ml a pronounced cell growth inhibition while at higher concentrations (0.266 and 0.443mg/ml) no cell proliferation was observed. CONCLUSIONS: The consumption of C. angustifolium herb can be advantageous, alongside with conventional breast cancer treatment
Internet: https://www.ncbi.nlm.nih.gov/pubmed/28282602
https://www.ncbi.nlm.nih.gov/pubmed/28282602
Affiliation(s): Biochemijos katedra
Gamtos mokslų fakultetas
Instrumentinės analizės atviros prieigos centras
Lietuvos sveikatos mokslų universitetas. Medicinos akademija
Lietuvos sveikatos mokslų universitetas. Medicinos akademija. Onkologijos institutas
Vytauto Didžiojo universitetas
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications

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