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Type of publication: Konferencijų tezės nerecenzuojamuose leidiniuose / Conference theses in non-peer-reviewed publications (T2)
Field of Science: Biologija / Biology (N010)
Author(s): Lipatova, Indrė;Supšinskaitė, Akvilina;Paulauskas, Algimantas
Title: Identification of Wolbachia endosymbiont in Fleas (Siphonaptera) by molecular methods
Is part of: Smart Bio: international conference, 18-20 May 2017, Kaunas : abstracts book. Kaunas : Vytautas Magnus University, 2017
Extent: p. 90-90
Date: 2017
Keywords: Wolbachia;Symbiont;Fleas
ISBN: 9786098104424
Abstract: Wolbachia pipientis is a vertically transmitted, gram-negative obligate intracellular symbiont of arthropods. The bacterium is best known for its ability to manipulate host reproductive biology where it can induce cytoplasmic incompatibility, parthenogenesis, feminization and male-killing. The aim of this study was to carry out molecular identification of Wolbachia species and determine their prevalence in fleas. Fleas were collected from small mammals in 2013-2014 from different regions of Lithuania. Ten flea species were collected: Megabothris walkeri, M. turbidus, Ctenophthalmus agyrtes, Ct. uncinatus, Ct. assimilis, Hystrichopsylla talpae, H. orientalis, Nosopsyllus fasciatus, Peromyscopsylla bidentata, Paleopsylla soricis. A total 152 fleas was screened for Wolbachia using wsp81F and wsp691R primers which amplified 590-632 bp fragment of the wsp gene. Wolbachia DNA was detected in 21,7% (33/152) fleas
Affiliation(s): Biologijos katedra
Gamtos mokslų fakultetas
Vytauto Didžiojo universitetas
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications

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