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Type of publication: conference paper
Type of publication (PDB): Konferencijų tezės nerecenzuojamuose leidiniuose / Conference theses in non-peer-reviewed publications (T2)
Field of Science: Biologija / Biology (N010)
Author(s): Degutytė-Fomins, Laima;Stefanskytė, Aistė;Šilkūnienė, Giedrė;Jasukaitienė, Aldona;Gulbinas, Antanas
Title: Hyperthermic chemotherapy effect on glutamate dehydrogenase activity in human ovarian, gastric and pancreatic cancer cells
Is part of: Smart Bio: international conference, 18-20 May 2017, Kaunas : abstracts book. Kaunas : Vytautas Magnus University, 2017
Extent: p. 87-87
Date: 2017
Keywords: Cancer cells;Cisplatin;Hyperthermia;Combinatory treatment
ISBN: 9786098104424
Abstract: Cancer is one of the leading causes of increasing morbidity and mortality worldwide. Tumours are characterized by high heterogeneity and transformation of metabolism to dysregulated Warburg-like glucose metabolism, dependence on glutaminolysis and fatty acid synthesis providing resistance for cancer treatments [1]. Cisplatin is clinically proven as mainline treatment of various cancers. To overcome the resistance cancer cells to cisplatin and reducing toxic side effects the hyperthermic intraperitoneal chemotherapy (HIPEC) is used [2]. Mitochondria in tumour cells perform the role of biosynthetic organelles and are to great extent dependent on activity glutamate dehydrogenase (GDH) that controls the supply of α- ketoglutarate from glutamine. In this study we investigated effects of mild hyperthermia 40oC and 43oC, cisplatin or combination of both treatments on GDH activity in three cancer cell lines (Ovcar-3, AGS and T3M4). The response to treatments and recovery of cell viability and GDH activity was strongly dependent on cell line. After hyperthermic treatment GDH activity in Ovcar-3 cell lines increased by 31% after heating 1 h at 40oC, and twice – at 43oC; in cell line T3M4 – by 31% after heating at 43oC; whereas GDH activity in AGS cell line was resistant to hyperthermia. During recovery GDH first undergoes inhibition (after 24h) and then is activated by 20-30% (after 48h) in all heated cells as compared to the control. Treatment with cisplatin alone strongly inhibited (by 40%) GDH activity only in Ovcar-3 cell line after 48h recovery, but did not affect GDH activity in T3M4 cell line or even stimulated GDH (by 20%) in AGS cell line. Hyperthermic chemotherapy inhibited GDH activity stronger in comparison to the cisplatin or hyperthermia as applied separately in all cancer cell lines, however the extent of inhibition different. The cell line Ovcar-3 was the most sensitive to the combinatory treatment. [...]
Affiliation(s): Biochemijos katedra
Gamtos mokslų fakultetas
Lietuvos sveikatos mokslų universitetas
Lietuvos sveikatos mokslų universitetas. Medicinos akademija. Virškinimo sistemos tyrimų institutas
Vytauto Didžiojo universitetas
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications

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