Dynamics of cell adhesion after alectroporation

Abstract

Cell is a semi open system which is constantly exchanging various molecules with it’s surrounding, however not all molecules enter or leave the cell. Cell electroporation (EP) is a process, when cells in suspension, monolayer or in a tissue are temporary permeabilized for various exogenous molecules by means of electric pulses, which are believed to create transient electropores in cells plasma membrane. Phenomena of EP facilitates ability to transfer molecules of interest that can vary in size of tens of kDa (electrotransfer, electrotransfection) into cells, or to remove from (electroextraction). Method of electroporation is already applicable in veterinary and clinics together for treatment of various oncological deseases (electrochemotherapy, electrogenetransfer). Furthermore, it was shown that EP cause delayed cell death, even though cells after reversible EP fully recover their plasma membrane. Dealayed cell death is assumed to appear due to extraction of vital biocompounds from cells (ions, RNA, DNA, proteins) during or post-electric fields while electropores are present. Intensity of letal changes in the cells caused by EP mainly depend on intensity, duration and number of pulses. Despite that, one of cells response as adhesion after EP is poorly invetigated. In this work we present methodology and results of CHO cells adhesion to growing dish dynamics after EP by means of intensity and number of electric pulses.