Cytotoxicity of vitamins C, K3 and their mixture for melanoma B16-F1 and hepatoma MH-22A cells In Vitro

Abstract

Melanoma – is one of the most prevalent skin cancer form, which is formed often by the sun's ultraviolet (UV) light and it damages DNA or do injuries for the skin cells. This cancer is one of the most diagnosed tumor in the USA. According to the Lithuanian Cancer Registry data, in 2012 the male and female skin cancer was one of the most abundant of the malignant oncological diseases locations (National Cancer Society, 2015). Concerning mortality, liver carcinoma is in the third place in the world. We need to find effective, non-invasive treatment that may cause cancer cells death except healthy tissue. Research shows that vitamin C (VC), vitamin (VK3) has anti-cancer features and using this compound, ratio 100:1 (VC:VK3), shows an increase of synergistic cytotoxicity. Cell’s damage are available of oxidative stress (caused by vitamins) during the reaction between free radicals compose reactive oxygen species (ROS) or their precursors, such as hydrogen peroxide. In this work, studied mentioned substrates cytotoxicity against tumor cells