Differentiations of chitin content and surface morphologies of chitins extracted from male and female grasshopper species

Kaya, Murat; Lelešius, Evaldas; Nagrockaitė, Radvilė; Sargin, Idris; Arslan, Gulsin; Mol, Abbas; Baran, Talat; Can, Esra; Bitim, Betul

Type of publication:

research article

Type of publication (PDB):

Straipsnis Clarivate Analytics Web of Science / Article in Clarivate Analytics Web of Science (S1)

Field of Science:

Biologija / Biology (N010)

Author(s):

Kaya, Murat;Lelešius, Evaldas;Nagrockaitė, Radvilė;Sargin, Idris;Arslan, Gulsin;Mol, Abbas;Baran, Talat;Can, Esra;Bitim, Betul

Title:

Differentiations of chitin content and surface morphologies of chitins extracted from male and female grasshopper species

Is part of:

PLoS ONE [elektroninis išteklius]. San Francisco, USA : Public Library of Science, 2015, vol. 10, iss. 1

Extent:

p. 1-14

Date:

2015

Keywords:

In this study, we used Fourier transform infrared spectroscopy (FT-IR), elemental analysis (EA), thermogravimetric analysis (TGA), X-ray diffractometry (XRD), and scanning electron microscopy (SEM) to investigate chitin structure isolated from both sexes of four grasshopper species. FT-IR, EA, XRD, and TGA showed that the chitin was in the alpha form. With respect to gender, two main differences were observed. First, we observed that the quantity of chitin was greater in males than in females and the dry weight of chitin between species ranged from 4.71% to 11.84%. Second, using SEM, we observed that the male chitin surface structure contained 25 - 90nm wide nanofibers and 90 - 250 nm nanopores, while no pores or nanofibers were observed in the chitin surface structure of the majority of females (nanofibers were observed only in M. desertus females). In contrast, the elemental analysis, thermal properties, and crystalline index values for chitin were similar in males and females. Also, we carried out enzymatic digestion of the isolated chitins using commercial chitinase from Streptomyces griseus. We observed that there were no big differences in digestion rate of the chitins from both sexes and commercial chitin. The digestion rates were for grasshoppers' chitins; 88.45-95.48% and for commercial chitin; 94.95%;Chitin

Internet:

http://www.plosone.org/article/fetchObject.action?uri=info:doi/10.1371/journal.pone.0115531&representation=PDF

Affiliation(s):

Gamtos mokslų fakultetas
Vytauto Didžiojo universitetas

Appears in Collections:

Universiteto mokslo publikacijos / University Research Publications

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