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Type of publication: conference paper
Type of publication (PDB): Tezės kituose recenzuojamuose leidiniuose / Theses in other peer-reviewed publications (T1e)
Field of Science: Biologija / Biology (N010)
Author(s): Nugaraitė, Dovilė;Mažeika, Vytautas;Paulauskas, Algimantas
Title: Identification and description of Isthmiophora melis (Digenea: Echinostomatidae) from mustelids in Lithuania: morphological and molecular data
Is part of: German society for parasitology : 27th annual meeting, 9–12 March 2016, Göttingen, Germany : program and abstracts. Göttingen : University of Göttingen, 2016
Extent: p. 156-156
Date: 2016
Keywords: Parasites;Morphological identification;Isthmiophora melis
Abstract: The Echinostomatidae Looss, 1899 is a large family of digenean whose adults occurs in small intestine of more than thirty species of vertebrates including humans. Morphological identification between members of genus Isthmiophora is difficult, due to high morphological similarities. In this case, identification based on molecular approaches is currently one of the most commonly used molecular methods in parasitology. The aim of this study was to identify the flukes of genus Isthmiophora from mustelids, using morphological and molecular data. The flukes were collected from American mink (Neovison vison) and European polecat (Mustela putorius) which were collected/hunted in different localities of Lithuania between 2013 and 2015. A total helminthological examination of individual organs of mustelids was carried out. Fluke specimens were processed for morphological studies following standard procedure of temporary and permanent preparations. The partial 18S rDNA, 28S rRNA, and ITS-1, 5.8S rRNA, and ITS-2 regions were used for molecular identification. According to morphological features, the flukes from both species of mustelids have short forebody (16.6% of body length), head collar reniform, small; collar spines 27 dorsal, four angle spines on each ventral lappet, longer than marginal spines; aboral collar spines (61×13) are slightly shorter than dorsal oral ones (67×14); post-testicular region is very long (38.8%), long armed cirrus, short uterus, large eggs (134×85). Molecular analysis revealed what most informative for species identification was ITS-1 region, which shared 100% homology with GenBank deposited I. melis isolates. On bases of morphological and molecular features flukes from mustelids were identified as I. melis (Schrank, 1788) Lühe, 1909
Affiliation(s): Biologijos katedra
Gamtos mokslų fakultetas
Vytauto Didžiojo universitetas
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications

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