Use this url to cite publication: https://hdl.handle.net/20.500.12259/54770
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Identification and description of Isthmiophora melis (Digenea: Echinostomatidae) from mustelids in Lithuania: morphological and molecular data
Type of publication
Tezės kitame recenzuojamame leidinyje / Theses in other peer-reviewed publication (T1e)
Title
Identification and description of Isthmiophora melis (Digenea: Echinostomatidae) from mustelids in Lithuania: morphological and molecular data
Is part of
German society for parasitology : 27th annual meeting, 9–12 March 2016, Göttingen, Germany : program and abstracts. Göttingen : University of Göttingen, 2016
Date Issued
Date Issued |
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2016 |
Publisher
Göttingen : University of Göttingen, 2016
Extent
p. 156-156
Field of Science
Abstract
The Echinostomatidae Looss, 1899 is a large family of digenean whose adults occurs in small intestine of more than thirty species of vertebrates including humans. Morphological identification between members of genus Isthmiophora is difficult, due to high morphological similarities. In this case, identification based on molecular approaches is currently one of the most commonly used molecular methods in parasitology. The aim of this study was to identify the flukes of genus Isthmiophora from mustelids, using morphological and molecular data. The flukes were collected from American mink (Neovison vison) and European polecat (Mustela putorius) which were collected/hunted in different localities of Lithuania between 2013 and 2015. A total helminthological examination of individual organs of mustelids was carried out. Fluke specimens were processed for morphological studies following standard procedure of temporary and permanent preparations. The partial 18S rDNA, 28S rRNA, and ITS-1, 5.8S rRNA, and ITS-2 regions were used for molecular identification. According to morphological features, the flukes from both species of mustelids have short forebody (16.6% of body length), head collar reniform, small; collar spines 27 dorsal, four angle spines on each ventral lappet, longer than marginal spines; aboral collar spines (61×13) are slightly shorter than dorsal oral ones (67×14); post-testicular region is very long (38.8%), long armed cirrus, short uterus, large eggs (134×85). Molecular analysis revealed what most informative for species identification was ITS-1 region, which shared 100% homology with GenBank deposited I. melis isolates. On bases of morphological and molecular features flukes from mustelids were identified as I. melis (Schrank, 1788) Lühe, 1909.
Type of document
type::text::conference output::conference proceedings::conference paper
Language
Anglų / English (en)
Coverage Spatial
Vokietija / Germany (DE)