Please use this identifier to cite or link to this item:https://hdl.handle.net/20.500.12259/54681
Type of publication: research article
Type of publication (PDB): Straipsnis Clarivate Analytics Web of Science / Article in Clarivate Analytics Web of Science (S1)
Field of Science: Biologija / Biology (N010)
Author(s): Šatkauskienė, Ingrida;Jarusevičiūtė, Simona;Baublys, Vykintas;Maheta, Mansi;Tubelytė-Kirdienė, Vaida
Title: Chitin hoops obtained from Ommatoiulus sabulosus (Diplopoda, Julidae) used for bovine serum albumin adsorption
Is part of: International journal of biological macromolecules. Amsterdam : Elsevier B.V., 2017, Vol. 94, B
Extent: p. 813-818
Date: 2016
Note: WOS:000390496100010
Keywords: 3D chitinas;Dviporiakojai;Chitinazės testas;Albuminas;3D chitin;Diplopod;Chitinase test;Albumin
Abstract: A new hoop shaped three dimensional chitin was obtained successfully from the body segment of a diplopod species (Ommatoiulus sabulosus) by following the procedure decolorization, demineralization and deproteinization. Purity of the hoop shaped three-dimensional chitin was proved by FT-IR analysis and chitinase digestive test. The important bands for -chitin such as 1654.2, 1619.7 and 1552 cm−1 were found after FT-IR analysis. And the chitinase digestive test revealed the purity of chitin (with digestion rate of 94.7%). SEM analysis showed that the chitin surface consisted of highly porous structure with nanofibers. Thermal stability of the hoop shaped chitin was recorded quite high (DTGmax = 383 ◦C). The nitrogen, carbon and hydrogen contents of the hoop shaped chitin were determined as 6.81%, 46.23% and 6.43% respectively. And also degree of acetylation (DA) of the chitin indicated the purity with 95.85%. Chitin hoops-BSA interaction was conducted at different pH, protein concentration and contacttime. This new type of three-dimensional chitin obtained from the diplopod body segments can find more effective applications in further studies comparing to the conventional dust forms
Internet: https://doi.org/10.1016/j.ijbiomac.2016.09.103
Affiliation(s): Biologijos katedra
Gamtos mokslų fakultetas
Vytauto Didžiojo universitetas
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications

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