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Type of publication: research article
Type of publication (PDB): Straipsnis Clarivate Analytics Web of Science / Article in Clarivate Analytics Web of Science (S1)
Field of Science: Biologija / Biology (N010)
Author(s): Cvirkaitė-Krupovič, Virginija;Krupovič, Mart;Daugelavičius, Rimantas;Bamford, Dennis H
Title: Calcium ion-dependent entry of the membrane-containing bacteriophage PM2 into its Pseudoalteromonas host
Is part of: Virology. San Diego : Elsevier B.V, 2010, Vol. 405, iss. 1
Extent: p. 120-128
Date: 2010
Keywords: Corticoviridae;Membrane-containing bacteriophage;Ca2+-dependent virus entry;Bacteriophage PM2;Cell lysis
Abstract: Marine bacteriophage PM2 infects gram-negative Pseudoalteromonas species and is currently the only assigned member of the Corticoviridae family. The icosahedral protein shell covers an internal protein-rich phage membrane that encloses the highly supercoiled dsDNA genome. In this study we investigated PM2 entry into the host. Our results indicate that PM2 adsorption to the host is dependent on the intracellular ATP concentration, while genome penetration through the cytoplasmic membrane depends on the presence of millimolar concentrations of calcium ions in the medium. In the absence of Ca2+ the infection is arrested at the entry stage but can be rescued by the addition of Ca2+. Interestingly, PM2 entry induces abrupt cell lysis if the host outer membrane is not stabilized by divalent cations. Experimental data described in this study in combination with results obtained previously allowed us to propose a sequential model describing the entry of bacteriophage PM2 into the host cells
Affiliation(s): Biochemijos katedra
Vilniaus universitetas
Vytauto Didžiojo universitetas
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications

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