Please use this identifier to cite or link to this item:https://hdl.handle.net/20.500.12259/54364
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dc.contributor.authorSaulis, Gintautas-
dc.contributor.authorLapė, Remigijus-
dc.contributor.authorPranevičiūtė, Rita-
dc.contributor.authorMickevičius, Donatas-
dc.coverage.spatialNL-
dc.date.accessioned2018-10-07T00:11:35Z-
dc.date.available2018-10-07T00:11:35Z-
dc.date.issued2005-
dc.identifier.issn15675394-
dc.identifier.otherVDU02-000006068-
dc.identifier.urihttps://doi.org/10.1016/j.bioelechem.2005.03.001-
dc.description.abstractThe change of the pH of a NaCl solution (139–149 mM NaCl) buffered with 5–15 mM sodium phosphates (pH 7.4) during electromanipulation was studied. It has been determined that an increase in the pH value of electroporation solution of a whole chamber volume, caused by the application of electric field pulses, commonly used in cell electromanipulation procedures, can exceed 1–2 pH units. Several materials for the cathode were tested. In all cases a stainless steel anode was utilized. The aluminum cathode gave a two-fold greater ΔpH in comparison with platinum, copper or stainless steel cathodes. In addition, a substantial release of aluminum (up to 1 mg/l) from the cathode was observed. It has also been found that the shift in pH depended on the medium conductivity: ΔpH of the solution, in which sucrose was substituted for NaCl, was about 5 times less. On the basis of the results obtained here, to avoid the plausible undesirable consequences of the cathodic electrolysis processes, in particular under the conditions of strong electric treatment, it could be recommended that chambers with aluminum electrodes not be utilized and one should use strongly buffered solutions of low conductivity and alternating current (sine or square wave) bipolar electric pulsesen
dc.description.sponsorshipGamtos mokslų fakultetas-
dc.description.sponsorshipVytauto Didžiojo universitetas-
dc.format.extentp. 101-108-
dc.language.isoen-
dc.relation.ispartofBioelectrochemistry. Amsterdam : Elsevier B.V., 2005, Vol. 67, iss. 1-
dc.relation.isreferencedbyScience Citation Index Expanded (Web of Science)-
dc.relation.isreferencedbyScienceDirect-
dc.relation.isreferencedbyScopus-
dc.relation.isreferencedbyMEDLINE-
dc.subjectCell electromanipulationen
dc.subjectElectroporationen
dc.subjectPH shiften
dc.subjectElectrolysis reactionsen
dc.subjectElectrodesen
dc.subject.classificationStraipsnis Clarivate Analytics Web of Science / Article in Clarivate Analytics Web of Science (S1)-
dc.subject.otherBiologija / Biology (N010)-
dc.titleChanges of the solution pH due to exposure by high-voltage electric pulsesen
dc.typeresearch article-
dc.identifier.doihttps://doi.org/10.1016/j.bioelechem.2005.03.001-
dc.identifier.isiWOS:000232022000012-
dcterms.bibliographicCitation55-
dc.date.updated2020-02-11T13:41Z-
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local.typeS-
item.grantfulltextopen-
item.fulltextWith Fulltext-
crisitem.author.deptBiologijos katedra-
crisitem.author.deptBiologijos katedra-
crisitem.author.deptGamtos mokslų fakultetas-
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications
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