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Type of publication: research article
Type of publication (PDB): Straipsnis Clarivate Analytics Web of Science / Article in Clarivate Analytics Web of Science (S1)
Field of Science: Biologija / Biology (N010)
Author(s): Saulis, Gintautas;Lapė, Remigijus;Pranevičiūtė, Rita;Mickevičius, Donatas
Title: Changes of the solution pH due to exposure by high-voltage electric pulses
Is part of: Bioelectrochemistry. Amsterdam : Elsevier B.V., 2005, Vol. 67, iss. 1
Extent: p. 101-108
Date: 2005
Keywords: Cell electromanipulation;Electroporation;PH shift;Electrolysis reactions;Electrodes
Abstract: The change of the pH of a NaCl solution (139–149 mM NaCl) buffered with 5–15 mM sodium phosphates (pH 7.4) during electromanipulation was studied. It has been determined that an increase in the pH value of electroporation solution of a whole chamber volume, caused by the application of electric field pulses, commonly used in cell electromanipulation procedures, can exceed 1–2 pH units. Several materials for the cathode were tested. In all cases a stainless steel anode was utilized. The aluminum cathode gave a two-fold greater ΔpH in comparison with platinum, copper or stainless steel cathodes. In addition, a substantial release of aluminum (up to 1 mg/l) from the cathode was observed. It has also been found that the shift in pH depended on the medium conductivity: ΔpH of the solution, in which sucrose was substituted for NaCl, was about 5 times less. On the basis of the results obtained here, to avoid the plausible undesirable consequences of the cathodic electrolysis processes, in particular under the conditions of strong electric treatment, it could be recommended that chambers with aluminum electrodes not be utilized and one should use strongly buffered solutions of low conductivity and alternating current (sine or square wave) bipolar electric pulses
Affiliation(s): Gamtos mokslų fakultetas
Vytauto Didžiojo universitetas
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications

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