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Type of publication: research article
Type of publication (PDB): Straipsnis Clarivate Analytics Web of Science / Article in Clarivate Analytics Web of Science (S1)
Field of Science: Biochemija / Biochemistry (N004)
Author(s): Tamulevičius, Tomas;Šeperys, Rimas;Andrulevičius, Mindaugas;Kopustinskas, Vitoldas;Meškinis, Šarūnas;Tamulevičius, Sigitas;Mikalayeva, Valeryia;Daugelavičius, Rimantas
Title: Application of holographic sub-wavelength diffraction gratings for monitoring of kinetics of bioprocesses
Is part of: Applied surface science. Amsterdam : Elsevier Science B.V., Vol. 258, no. 23 (2012)
Extent: p. 9292-9296
Date: 2012
Keywords: Refractive index sensor;Laser interference lithography;Sub-wavelength diffraction grating;SiOx doped DLC;Lysozyme;B. subtilis
Abstract: In this work we present a refractive index (RI) sensor based on a sub-wavelength holographic diffraction grating. The sensor chip was fabricated by dry etching of the finely spaced (d = 428 nm) diffraction grating in SiOx doped diamond like carbon (DLC) film. It is shown that employing a fabricated sensor chip, and using the proposed method of analysis of data, one can inspect kinetics of processes in liquids occurring in the vicinity of the grating surface. The method is based on the spectral composition analysis of polarized polychromatic light reflected from the sub-wavelength diffraction grating. The RI measurement system was tested with different model liquid analytes including 25 wt.%, 50 wt.% sugar water solutions, 10 °C, 50 °C distilled water, also Gram-positive bacteria Bacillus subtilis interaction with ion-permeable channels forming antibiotic gramicidin D and a murolytic enzyme lysozyme. Analysis of the data set of specular reflection spectra enabled us to follow the kinetics of the RI changes in the analyte with millisecond resolution. Detectable changes in the effective RI were not worse than Δn = 10−4.ctural transformations are correlated with oxygen contamination and modify hydrogen storage properties
Affiliation(s): Biochemijos katedra
Kauno technologijos universitetas
Vytauto Didžiojo universitetas
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications

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