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Type of publication: research article
Type of publication (PDB): Straipsnis Clarivate Analytics Web of Science / Article in Clarivate Analytics Web of Science (S1)
Field of Science: Biochemija / Biochemistry (N004)
Author(s): Hennessy, Tom P;Quaglia, M;Kornyšova, Olga;Grimes, Brian A;Lubda, Dieter;Unger, Klaus K
Title: Dynamic electric field assisted multi-dimensional liquid chromatography of biological samples
Other Title: Biologinių bandinių dinaminio elektrinio lauko palaikoma multidimensinė skysčių chromatografija
Is part of: Journal of chromatography. B. Amsterdam : Elsevier B.V., 2005, Vol. 817, iss. 1
Extent: p. 127-135
Date: 2005
Keywords: Multidimensional liquid chromatography;Size exclusion chromatography;Strong cation exchange chromatography;Tailor-made sorbents;Monolithic capillary columns;On-line sample clean-up;Polarity reversal;Human hemofiltrate;Affinity chromatography
Abstract: Complex biological samples require very high resolution separation strategies. The platform introduced here capitalises on the hyphenation of liquid chromatographic (LC) and electric potential gradient electrochromatographic multi-dimensional separation genres. First-dimension selectivity is provided by simultaneous size exclusion (SEC) and strong cation exchange (SCX) chromatography modes, while the second dimension comprises reversed phase (RP) characteristics in a dynamic (time-variant) electric field. The time-variant potential gradient with reversal of polarity is applied across the second dimension monolithic capillary throughout the duration of the solvent strength gradient elution. Hence, the platform offers comprehensive on-line sample clean-up (matrix depletion, analyte enrichment), fractionation (first dimention LC), and separation (second dimension LC) with the prospect of altering selectivity via polarity reversal dynamic electric field tuning
Affiliation(s): Vytauto Didžiojo universitetas
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications

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