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Type of publication: Straipsnis Clarivate Analytics Web of Science ar/ir Scopus / Article in Clarivate Analytics Web of Science or / and Scopus (S1)
Field of Science: Biochemija / Biochemistry (N004)
Author(s): Tetala, K. Kishore R;Chen, Bo;Visser, Gerben M;Maruška, Audrius;Kornyšova, Olga;Beek, Teris A. van;Sudhölter, Ernst J.R
Title: Preparation of a monolithic capillary column with immobilized α-mannose for affinity chromatography of lectins
Other Title: Monolitinių kapiliarinių kolonėlių su imobilizuota α-manoze, skirtų afininei lektinų chromatografijai, sintezė
Is part of: Journal of biochemical and biophysical methods. Amsterdam : Elsevier B.V., Vol. 70, iss. 1 (2007)
Extent: p. 63-69
Date: 2007
Keywords: Capillary column;Continuous bed;Monolith;Porosity;Affinity chromatography;Lectins;Scanning electron microscopy;Inverse size exclusion chromatography;Frontal analysis
Abstract: A simple method for the preparation of an affinity monolithic (also called continuous bed) capillary column for α-mannose-specific lectins is described. 2-Hydroxyethyl methacrylate in combination with (+)-N,N´-diallyltartardiamide (DATD) and piperazine diacrylamide (PDA, 1,4-bisacryloyl-piperazine) as crosslinkers, were used as monomers for the monolith. After oxidation of DATD with periodate, α-mannose with spacer was bound to the aldehyde groups of the polymeric skeleton via reductive amination to form an affinity column for the separation, enrichment or binding studies of mannose-specific lectins. The permeability of the column was excellent. The porosity of the monolith was investigated by scanning electron microscope (SEM) and inverse size exclusion chromatography (ISEC). The affinity of the monolith was evaluated by frontal analysis (FA) and fluorescence microscopy (FM) using fluorescently labeled concanavalin (Con A). Frontal affinity chromatography showed a specific interaction of two different lectins with the α-mannose-modified monolith. According to FM the affinity sites were evenly distributed over the monolithic bed
Affiliation(s): Vytauto Didžiojo universitetas
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications

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