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Type of publication: research article
Type of publication (PDB): Straipsnis Clarivate Analytics Web of Science / Article in Clarivate Analytics Web of Science (S1)
Field of Science: Chemija / Chemistry (N003)
Author(s): Kornyšova, Olga;Maruška, Audrius;Owens, Paul K;Erickson, M
Title: Non-particulate (continuous bed or monolithic) acrylate-based capillary columns for reversed-phase liquid chromatography and electrochromatography
Other Title: Nebirios (ištisinės arba monolitinės) akrilatinės kapiliarinės kolonėlės, skirtos atvirkščių fazių skysčių chromatografijai ir elektrochromatografijai
Is part of: Journal of chromatography. A. Amsterdam : Elsevier B.V., 2005, Vol. 1071, iss. 1-2
Extent: p. 171-178
Date: 2005
Keywords: Reversed-phase capillary electrochromatography;CEC;Capillary liquid chromatography;Inverse size exclusion chromatography;Continuous beds;Monoliths;Hydrophobicity;Porosimetry
Abstract: Three approaches are described to synthesize acrylic non-particulate beds (also called continuous beds or monoliths) in aqueous polymerization media for reversed-phase capillary liquid chromatography/electrochromatography. In the first, hexyl acrylate comonomer was dissolved together with water soluble polar comonomers using a non-ionic detergent. In the second, a new alkyl ammonium salt comonomer, (3-allylamino-2-hydroxypropyl)dodecyldimethylammonium chloride was used, which is water soluble and has detergent properties itself. The alkyl group of this comonomer provides hydrophobicity while the ionic groups generate electroosmosis in the non-particulate bed. In the third approach, the alkyl comonomer was used as a detergent to dissolve another hydrophobic comonomer in an aqueous polymerization medium. All three approaches were evaluated with respect to hydrophobicity, efficiency and electroosmotic properties of the beds. Hydrophobicity expressed as methylene group selectivity for the three types of the beds in 50% methanol mobile phase was 1.86, 1.16 and 1.78, electroosmotic mobility −5.14 × 10−5, 6.89 × 10−5 and 6.37 × 10−5 cm2 V−1 s−1 and efficiency for the retained compound (methylparabene) 67,000, 93,000 and 110,000 plates m−1 correspondingly. The columns were tested using pressure driven capillary chromatography and capillary electrochromatography. The influence of polymerization temperature on hydrodynamic permeability, separation impedance and inverse size exclusion porosimetry characteristics were used to evaluate the separation columns. The increase of the polymerization temperature resulted higher permeability of the bed, separation impedance and lower polymeric skeleton porosity. Further characterisation was provided by examining the separation efficiency observed for a series of benzoic acid esters and alkyl parabens
Affiliation(s): Vytauto Didžiojo universitetas
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications

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