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Type of publication: research article
Type of publication (PDB): Straipsnis Clarivate Analytics Web of Science / Article in Clarivate Analytics Web of Science (S1)
Field of Science: Biologija / Biology (N010);Chemijos inžinerija / Chemical engineering (T005)
Author(s): Gianulis, Elena C;Labib, Chantelle;Saulis, Gintautas;Novickij, Vitalij;Pakhomova, Olga N;Pakhomov, Andrei G
Title: Selective susceptibility to nanosecond pulsed electric field (nsPEF) across different human cell types
Is part of: Cellular and molecular life sciences : CMLS. Basel : Springer International Publishing, 2017, Vol. 74, iss. 9
Extent: p. 1741-1754
Date: 2017
Keywords: Nanosekundiniai elektriniai impulsai;Mikrosekundiniai elektriniai impulsai;Citotoksiškumas;Navikų abliacija;Elektroporacija;Nanosecond electric pulses;Microsecond electric pulses;Cytotoxicity;Cancer ablation;Electroporation
Abstract: Tumor ablation by nanosecond pulsed electric fields (nsPEF) is an emerging therapeutic modality. We compared nsPEF cytotoxicity for human cell lines of cancerous (IMR-32, Hep G2, HT-1080, and HPAF-II) and non-cancerous origin (BJ and MRC-5) under strictly controlled and identical conditions. Adherent cells were uniformly treated by 300-ns PEF (0–2000 pulses, 1.8 kV/ cm, 50 Hz) on indium tin oxide-covered glass coverslips, using the same media and serum. Cell survival plotted against the number of pulses displayed three distinct regions (initial resistivity, logarithmic survival decline, and residual resistivity) for all tested cell types, but with differences in LD50 spanning as much as nearly 80-fold. The non-cancerous cells were less sensitive than IMR-32 neuroblastoma cells but more vulnerable than the other cancers tested. The cytotoxic efficiency showed no apparent correlation with cell or nuclear size, cell morphology, metabolism level, or the extent of membrane disruption by nsPEF. Increasing pulse duration to 9 ls (0.75 kV/cm, 5 Hz) produced a different selectivity pattern, suggesting that manipulation of PEF parameters can, at least for certain cancers, overcome their resistance to nsPEF ablation. Identifying mechanisms and cell markers of differential nsPEF susceptibility will critically contribute to the proper choice and outcome of nsPEF ablation therapies
Affiliation(s): Biologijos katedra
Vytauto Didžiojo universitetas
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications

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