Please use this identifier to cite or link to this item:https://hdl.handle.net/20.500.12259/54268
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dc.contributor.authorBalčiūnaitė, Gabrielė-
dc.contributor.authorJuodsnukytė, Jovita-
dc.contributor.authorSavickas, Arūnas-
dc.contributor.authorRagažinskienė, Ona-
dc.contributor.authorSiatkutė, Luka-
dc.contributor.authorŽvirblytė, Gitana-
dc.contributor.authorMistinienė, Edita-
dc.contributor.authorSavickienė, Nijolė-
dc.coverage.spatialDE-
dc.date.accessioned2018-10-07T00:07:17Z-
dc.date.available2018-10-07T00:07:17Z-
dc.date.issued2015-
dc.identifier.issn13300075-
dc.identifier.otherVDU02-000018867-
dc.identifier.urihttps://www.degruyter.com/downloadpdf/j/acph.2015.65.issue-4/acph-2015-0036/acph-2015-0036.xml-
dc.identifier.urihttp://hrcak.srce.hr/index.php?show=toc&id_broj=11567-
dc.descriptionISSN (Online) 1846-9558-
dc.description.abstractEchinacea purpurea (L.) Moench, a member of the Asteraceae family, is a plant rich in flavonoids, essential oils, phenolic compounds, saponins, polysaccharides and glycoproteins. The aim of the study was to evaluate the protein content in dried roots of Echinacea purpurea (L.) Moench after homogenization of roots with liquid nitrogen, extraction in 0.01 mol L-1 phosphate-buffered saline (PBS) and purification followed by fractionation of proteins using gel filtration chromatography. Total concentration of proteins was measured using the Bradford method, and evaluation of the molecular mass of proteins was accomplished by applying the SDS-PAGE gel electrophoresis. The Bradford assay revealed that the highest concentration of proteins in fractions collected after gel filtration chromatography was 4.66–6.07 mg mL-1. Glycoproteins, alkamides and polysaccharides in roots of Echinacea purpurea (L.) Moench are chemical compounds that are responsible for their immunomodulatory properties. However, information about the difference of protein contents in fresh and dried roots of E. purpurea is insufficienten
dc.description.sponsorshipBotanikos sodas-
dc.description.sponsorshipLietuvos sveikatos mokslų universitetas-
dc.description.sponsorshipLietuvos sveikatos mokslų universitetas. Medicinos akademija-
dc.description.sponsorshipVilniaus universitetas. Biotechnologijų centras-
dc.description.sponsorshipVytauto Didžiojo universitetas-
dc.format.extentp. 473-479-
dc.language.isoen-
dc.relation.ispartofActa pharmaceutica : the journal of Croatian pharmaceutical society. Berlin : Walter De Gruyter, 2015, Vol. 65, iss. 4-
dc.relation.isreferencedbyScience Citation Index Expanded (Web of Science)-
dc.relation.isreferencedbyMEDLINE Complete (EBSCO)-
dc.relation.isreferencedbyDe Gruyter-
dc.subjectEchinacea purpurea (L.) Moenchlt
dc.subjectProteinailt
dc.subjectGelio filtravimo chromatografijalt
dc.subjectEchinacea purpurea (L.) Moenchen
dc.subjectProteinsen
dc.subjectGel filtration chromatographyen
dc.subjectSDS-PAGEen
dc.subject.classificationStraipsnis Clarivate Analytics Web of Science / Article in Clarivate Analytics Web of Science (S1)-
dc.subject.otherFarmacija / Pharmacy (M003)-
dc.titleFractionation and evaluation of proteins in roots of Echinacea purpurea (L.) Moenchen
dc.typeresearch article-
dc.identifier.doihttps://doi.org/10.1515/acph-2015-0036-
dc.identifier.isiWOS:000366680500010-
dcterms.bibliographicCitation14-
dc.date.updated2020-01-29T15:16Z-
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local.typeS-
item.fulltextNo Fulltext-
item.grantfulltextnone-
crisitem.author.deptVaistinių ir prieskon. augalų kol. Sektorius-
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications
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