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Type of publication: research article
Type of publication (PDB): Straipsnis Clarivate Analytics Web of Science / Article in Clarivate Analytics Web of Science (S1)
Field of Science: Farmacija / Pharmacy (M003)
Author(s): Balčiūnaitė, Gabrielė;Juodsnukytė, Jovita;Savickas, Arūnas;Ragažinskienė, Ona;Siatkutė, Luka;Žvirblytė, Gitana;Mistinienė, Edita;Savickienė, Nijolė
Title: Fractionation and evaluation of proteins in roots of Echinacea purpurea (L.) Moench
Is part of: Acta pharmaceutica : the journal of Croatian pharmaceutical society. Berlin : Walter De Gruyter, 2015, Vol. 65, iss. 4
Extent: p. 473-479
Date: 2015
Note: ISSN (Online) 1846-9558
Keywords: Echinacea purpurea (L.) Moench;Proteinai;Gelio filtravimo chromatografija;Echinacea purpurea (L.) Moench;Proteins;Gel filtration chromatography;SDS-PAGE
Abstract: Echinacea purpurea (L.) Moench, a member of the Asteraceae family, is a plant rich in flavonoids, essential oils, phenolic compounds, saponins, polysaccharides and glycoproteins. The aim of the study was to evaluate the protein content in dried roots of Echinacea purpurea (L.) Moench after homogenization of roots with liquid nitrogen, extraction in 0.01 mol L-1 phosphate-buffered saline (PBS) and purification followed by fractionation of proteins using gel filtration chromatography. Total concentration of proteins was measured using the Bradford method, and evaluation of the molecular mass of proteins was accomplished by applying the SDS-PAGE gel electrophoresis. The Bradford assay revealed that the highest concentration of proteins in fractions collected after gel filtration chromatography was 4.66–6.07 mg mL-1. Glycoproteins, alkamides and polysaccharides in roots of Echinacea purpurea (L.) Moench are chemical compounds that are responsible for their immunomodulatory properties. However, information about the difference of protein contents in fresh and dried roots of E. purpurea is insufficient
Affiliation(s): Botanikos sodas
Lietuvos sveikatos mokslų universitetas
Lietuvos sveikatos mokslų universitetas. Medicinos akademija
Vilniaus universitetas. Biotechnologijų centras
Vytauto Didžiojo universitetas
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications

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