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Type of publication: research article
Type of publication (PDB): Straipsnis Clarivate Analytics Web of Science / Article in Clarivate Analytics Web of Science (S1)
Field of Science: Biologija / Biology (N010)
Author(s): Maciulevičius, Martynas;Tamošiūnas, Mindaugas;Jakštys, Baltramiejus;Jurkonis, Rytis;Venslauskas, Mindaugas Saulius;Šatkauskas, Saulius
Title: Investigation of microbubble cavitation-induced calcein release from cells in vitro
Is part of: Ultrasound in medicine and biology. New York, NY : Elsevier, 2016, vol. 42, iss. 12
Extent: p. 2990-3000
Date: 2016
Keywords: Sonoporacija;Kalceinas;Inercinės kavitacijos dozė;Ateniuacija;Mikroburbulai;Sonoporation;Calcein;Extraction;Inertial cavitation dose;Attenuation;Microbubble
Abstract: In the present study, microbubble (MB) cavitation signal analysis was performed together with calcein release evaluation in both pressure and exposure duration domains of the acoustic field. A passive cavitation detection system was used to simultaneously measure MB scattering and attenuation signals for subsequent extraction efficiency relative to MB cavitation activity. The results indicate that the decrease in the efficiency of extraction of calcein molecules from Chinese hamster ovary cells, as well as cell viability, is associated with MB cavitation activity and can be accurately predicted using inertial cavitation doses up to 0.18 V × s (R2 > 0.9, p < 0.0001). No decrease in additional calcein release or cell viability was observed after complete MB sonodestruction was achieved. This indicates that the optimal exposure duration within which maximal sono-extraction efficiency is obtained coincides with the time necessary to achieve complete MB destruction. These results illustrate the importance of MB inertial cavitation in the sono-extraction process. To our knowledge, this study is the first to (i) investigate small molecule extraction from cells via sonoporation and (ii) relate the extraction process to the quantitative characteristics of MB cavitation acoustic spectra
Affiliation(s): Biologijos katedra
Gamtos mokslų fakultetas
Kauno technologijos universitetas
Vytauto Didžiojo universitetas
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications

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