Please use this identifier to cite or link to this item:https://hdl.handle.net/20.500.12259/52955
Type of publication: conference paper
Type of publication (PDB): Konferencijų tezės nerecenzuojamuose leidiniuose / Conference theses in non-peer-reviewed publications (T2)
Field of Science: Farmacija / Pharmacy (M003)
Author(s): Balčiūnaitė, Gabrielė;Žvirblis, Gintautas;Ragažinskienė, Ona;Savickienė, Nijolė
Title: Purification of purple coneflower root hemagglutinin by affinity chromatography
Is part of: Science and practice 2016: the 7th international pharmaceutical conference, dedicated to the 240th anniversary of prof. Johann Friedrich Wolfgang, October 20-21, 2016, Kaunas, Lithuania : book of abstracts. Kaunas : Lithuanian university of Health Sciences, 2016
Extent: p. 16-17
Date: 2016
Keywords: Echinacea;Plant proteins;Hemagglutinins;Chromatography, affinity
ISBN: 9789955154549
Abstract: Lecitins or hemagglutinins are non-immune origin glycoproteins, which can bind carbohydrate structures in specific and reversible manner. This particular feature enables lectin purification by affinity chromatography from complex protein samples. Proteins from Echinerea purpurea L. (Moench) roots haven't been investigated precisely, even though this herb is widely used in traditional medicine. Aim of experiment: To develop hemagglutinin from purple coneflower root purification by affinity chromatography. Experiment tasks: 1. Define carbohydrate affinity to hemagglutinins in purple coneflower root protein extracts; 2. Modify affinity matrix for lectin purification from purple coneflower root protein mixture; 3. Purity hemagglutinins from purple coneflower roots. Materials and methods: 1. To ascertain carbohydratte specificity of hemagglutinin component in crude purple coneflower root extract was determined by hemagglutination inhibition reaction in V bottomed microtiter plates. Twofold serial dilutions of crude protein extract were made and the sample was mixed and incubated for 1 h with equal volume of carbohydrate samples: 3 pentoses: D-arabinose, D-ribose, D-xylose, 5 hexoses and their derivates: D-galactose, D-mannose, D-glucose, Acetyl glucosamine, N-Acetyl glucosamine, 2 disaccharides D-lactose, D-maltose. [...]
Internet: https://hdl.handle.net/20.500.12259/52955
Affiliation(s): Botanikos sodas
Lietuvos sveikatos mokslų universitetas. Medicinos akademija
Vilniaus universitetas. Biotechnologijos institutas
Vytauto Didžiojo universitetas
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications

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