Please use this identifier to cite or link to this item:https://hdl.handle.net/20.500.12259/52108
Type of publication: Konferencijų tezės nerecenzuojamuose leidiniuose / Conference theses in non-peer-reviewed publications (T2)
Field of Science: Biochemija / Biochemistry (N004)
Author(s): Snitka, Valentinas;Ramanauskaitė, Lina;Xu, Huizhong;Gadisauskas, Tomas;Žūkienė, Rasa
Title: Novel plasmonic probes for Tip-Enhanced Raman spectroscopy
Is part of: ImagineNano 2015 : 3rd edition of the largest European event in Nanoscience & Nanotechnology, March 10-13, 2015, Bilbao, Spain. Madrid : Phantoms foundation, 2015
Extent: p. 1-1
Date: 2015
Keywords: TERS;Alfa sinukleinas;TERS;Alpha-synuclein
Abstract: Hybridization of Atomic force microscopy with plasmonic probes stimulated the development of the several new spectroscopic techniques. Tip-Enhanced Raman Scattering (TERS) is the technique utilizing a special AFM probe (nano-antenna) to localize light at the nanometer scale area near the probe apex. TERS has emerged as a potentially powerful nanochemical analysis tool [1]. However, questions about the reproducibility of TERS data still remain a challenge [2]. The TERS data of biomolecules obtained in liquid open even more questions. A deep integration of AFM with confocal Raman microscopy is required for successful TERS experiment. In this work we investigated the application of novel AFM plasmonic probes for TERS fingerprinting of proteins ( α –synuclein) and monitoring the structural changes during the protein interaction with nanoparticles. We used a commercial novel AFM-TERS probes (NT-MDT Inc.) and home made AFM-TERS probes made by chemical silver deposition on socalled “Top Visual” AFM Si cantilevers (NT-MDT Inc.). Top-illumination AFM-Tip-enhanced Raman scattering system adapted on an upright optical microscope have been used in our experiments. Simulation of localized plasmon electromagnetic field distribution between the tip and substrate was done using COMSOL software EM package. We evaluated the Raman enhancement spectra as a function of experimental variables such as excitation power, acquisition time, molecules type and substrate. Based on the spectra obtained, the peak positions, number of bands, peak intensity ratios, and comparability to reference micro-Raman data are discussed. This study demonstrated the suitability of investigated cantilevers to be used for biological TERS applications
Internet: http://www.phantomsnet.net/imaginenano/Abstracts2015/PPM2015_Snitka_Valentinas_vsnitka@ktu.lt_ImagineNano2015_Abstract_Valentinas%20Snitka.pdf
http://www.phantomsnet.net/imaginenano/Abstracts2015/PPM2015_Snitka_Valentinas_vsnitka@ktu.lt_ImagineNano2015_Abstract_Valentinas%20Snitka.pdf
Affiliation(s): Biochemijos katedra
Gamtos mokslų fakultetas
Kauno technologijos universitetas
Vytauto Didžiojo universitetas
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications

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