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dc.contributor.authorRilskytė, Karolina-
dc.contributor.authorNaučienė, Zita-
dc.contributor.authorSnitka, Valentinas-
dc.contributor.authorŽūkienė, Rasa-
dc.description.abstractZinc oxide nanoparticles (ZnO NPs) are increasingly applied in a diverse array of industrial and commercial products. In the biological milieu a protein corona is formed on the surface of NPs. The protein corona changes the mode of NPs interaction with cells, organelles and molecules resulting in a different biological effect of NPs as compared to plain NPs. Bovine serum albumin (BSA) and zinc oxide nanoparticles (ZnO NPs) were chosen in this study as a model system to investigate NPs-protein corona complex toxicity to cells and isolated mitochondria. In the cellular experimental model plain 20 nm ZnO NPs (ZnO) and ZnO NPs coated with bovine serum albumin (ZnO-BSA) were tested for citotoxicity and ROS generation in rabbit myoblast (Fr2) and human breast carcinoma (MX-1) cell lines. Both NPs types were cytotoxic with similar dependency on concentration, however, NPs were appr. 10% less toxic to Fr2 at high concentration (200 μg/ml) as compared to MX-1 and ZnO-BSAwere less toxic than ZnO NPs in the concentration range of 150-200 μg/ml. ZnO-BSA NPs haven’t induced ROS generation in none of cell line. The minimum ZnO concentration that induced ROS generation in MX-1 was lower as compared to Fr2 – 20 and 50 μg/ml, respectively. The treatment of mitochondria with ZnO NPs had an unexpected effect: ZnO-BSA NPs impaired stronger the mitochondrial respiration in metabolic state 3 and uncoupled state and induced more pronounced mitochondrial swelling than ZnO NPs meaning the opening of the permeability transition pore. ZnO-BSA NPs inhibited state 3 respiration rate by 50% at the concentration of 0.25 μg/ml, whereas ZnO NPs – at 0.40 μg/ml. Moreover, the site of inhibition in oxidative phosphorylation system was different for plain and BSA-coated NPs: the main effect of ZnO NPs was on phosphorylation subsystem whereas the main effect of ZnO-BSA NPs was on respiratory subsystem. [...]en
dc.description.sponsorshipBiochemijos katedra-
dc.description.sponsorshipGamtos mokslų fakultetas-
dc.description.sponsorshipKauno technologijos universitetas-
dc.description.sponsorshipVytauto Didžiojo universitetas-
dc.format.extentp. 82-82-
dc.relation.ispartofThe vital nature sign [elektroninis išteklius] : 9th international scientific conference : abstract book. Kaunas : Vytautas Magnus university, 2015, [no. 9]-
dc.subjectZnO nanoparticlesen
dc.subjectProtein coronaen
dc.subject.classificationTezės kituose recenzuojamuose leidiniuose / Theses in other peer-reviewed publications (T1e)-
dc.subject.otherChemija / Chemistry (N003)-
dc.titleSerum albumin corona on ZnO NPs has opposite effect in cellular and mitochondrial toxicity experimental modelsen
dc.typeconference paper-
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Appears in Collections:Universiteto mokslo publikacijos / University Research Publications
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