Use this url to cite publication: https://hdl.handle.net/20.500.12259/51703
Publication type
type::text::periodical::journal::contribution to journal::journal article::research article
Type of publication (PDB)
Straipsnis Clarivate Analytics Web of Science / Article in Clarivate Analytics Web of Science (S1)
Title
DRG axon elongation and growth cone collapse rate induced by Sema3A are differently dependent on NGF concentration
Is part of
Cellular and molecular neurobiology. New York : Springer, Vol. 34, 2014
Extent
p. 289-296
Publisher
New York : Springer
Publisher (trusted)
Springer
Date Issued
2014
Description
ISSN: 1573-6830 (electronic version)
ISSN (of the container)
0272-4340
DOI
https://doi.org/10.1007/s10571-013-0013-x
WOS
WOS:000332948800014
Other Identifier(s)
VDU02-000014615
Abstract
Regeneration of embryonic and adult dorsal root ganglion (DRG) sensory axons is highly impeded when they encounter neuronal growth cone-collapsing factor semaphorin3A (Sema3A). On the other hand, increasing evidence shows that DRG axon's regeneration can be stimulated by nerve growth factor (NGF). In this study, we aimed to evaluate whether increased NGF concentrations can counterweight Sema3A-induced inhibitory responses in 15-day-old mouse embryo (E15) DRG axons. The DRG explants were grown in Neurobasal-based medium with different NGF concentrations ranging from 0 to 100 ng/mL and then treated with Sema3A at constant 10 ng/mL concentration. To evaluate interplay between NGF and Sema3A number of DRG axons, axon outgrowth distance and collapse rate were measured. We found that the increased NGF concentrations abolish Sema3A-induced inhibitory effect on axon outgrowth, while they have no effect on Sema3A-induced collapse rate.
Bibliographic Details
59
Coverage Spatial
US
Language
Anglų / English (en)