Use this url to cite publication: https://hdl.handle.net/20.500.12259/51703
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DRG axon elongation and growth cone collapse rate induced by Sema3A are differently dependent on NGF concentration
Type of publication
Straipsnis Web of Science ir Scopus duomenų bazėje / Article in Web of Science and Scopus database (S1)
Title
DRG axon elongation and growth cone collapse rate induced by Sema3A are differently dependent on NGF concentration
Is part of
Cellular and molecular neurobiology. New York : Springer, Vol. 34, 2014
Date Issued
Date Issued |
---|
2014 |
Publisher
New York : Springer
Publisher (trusted)
Extent
p. 289-296
Field of Science
Abstract
Regeneration of embryonic and adult dorsal root ganglion (DRG) sensory axons is highly impeded when they encounter neuronal growth cone-collapsing factor semaphorin3A (Sema3A). On the other hand, increasing evidence shows that DRG axon's regeneration can be stimulated by nerve growth factor (NGF). In this study, we aimed to evaluate whether increased NGF concentrations can counterweight Sema3A-induced inhibitory responses in 15-day-old mouse embryo (E15) DRG axons. The DRG explants were grown in Neurobasal-based medium with different NGF concentrations ranging from 0 to 100 ng/mL and then treated with Sema3A at constant 10 ng/mL concentration. To evaluate interplay between NGF and Sema3A number of DRG axons, axon outgrowth distance and collapse rate were measured. We found that the increased NGF concentrations abolish Sema3A-induced inhibitory effect on axon outgrowth, while they have no effect on Sema3A-induced collapse rate.
Type of document
type::text::journal::journal article::research article
Language
Anglų / English (en)
Coverage Spatial
Jungtinės Amerikos Valstijos / United States of America (US)
Description
ISSN: 1573-6830 (electronic version)