Use this url to cite publication: https://hdl.handle.net/20.500.12259/51379
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SNX482 inhibits semaphorin 3A induced sensory axon growth cone collapse
Type of publication
Tezės Clarivate Analytics Web of Science / Theses in Clarivate Analytics Web of Science (T1a)
Author(s)
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Kauno medicinos universiteto Kardiologijos institutas
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Jover, Emmanuel
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INCI–UPR-CNRS 3212, Neurotransmission et secretion neuroendocrine, Strasbourg, France
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Bagnard, Dominique
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Inserm u1109, MN3t lab, Strasbourg, France
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Title
SNX482 inhibits semaphorin 3A induced sensory axon growth cone collapse
Publisher
Hoboken: Wiley-Blackwell
Publisher (trusted)
Wiley Blackwell
Date Issued
2015
Extent
p. 169-169
Is part of
FEBS journal. Hoboken: Wiley-Blackwell, 2015, vol. 282, suppl. 1
Description
Abstracts of special issue: 40th FEBS congress The Biochemical Basis of Life, Berlin, Germany, July 4-9, 2015. P21-006-SP
Field of Science
Abstract
During axon navigation and regeneration after injuries a guidance molecule semaphorin 3A (sema3A) is one of the principalproteins in repulsing axons and inhibiting their growth. It is nowknown that calcium (Ca2+) is important in axon response toattractive guidance cues. On the other hand it still remains elusive if this is also true for repulsive guidance cue sema3A. In thisstudy intracellular calcium ([Ca2 + ]i) imaging was used to evaluate if sema3A-induced growth cone collapse is Ca2+dependent.[Ca2+]iimaging with ratiometric dye Fura-2 AM showed Ca2+increase in E15 mice dorsal root ganglia neuron growth cones inresponse to sema3A. Therefore Sema3A effects on growth conesafter modifying [Ca2+]iand [Ca2+]echannels that we have shownare expressed in E15 mouse embryos were evaluated. Results ofour study showed that sema3A indeed increased growth cone collapse rate that was blocked by the non-selective R- and T- typeCa2+channel inhibitor NiCl2and by the selective R-type Ca2+channel inhibitor SNX482. Ca2+channel inhibitors used havedecreased the sema3A-induced [Ca2+]iconcentration elevation. Results of this study demonstrated that sema3A-induced growth cone collapses are related to the increase in [Ca2+]iconcentrationin sensory axon growth cones and this is mediated through the R-type calcium channels.
Type of document
type::text::conference object::conference proceedings::conference paper
ISSN (of the container)
1742-464X
WOS
WOS:000362570603033
Other Identifier(s)
VDU02-000019075
Coverage Spatial
Jungtinės Amerikos Valstijos / United States of America (US)
Language
Anglų / English (en)