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Type of publication: Straipsnis Clarivate Analytics Web of Science ar/ir Scopus / Article in Clarivate Analytics Web of Science or / and Scopus (S1)
Field of Science: Agronomija / Agronomy (A001)
Author(s): Aleliūnas, Andrius;Jonavičienė, Kristina;Statkevičiūtė, Gražina;Vaitiekūnaite, Dorotėja;Kemešytė, Vilma;Lūbberstedt, T;Brazauskas, Gintaras
Title: Association of single nucleotide polymorphisms in LpIRI1 gene with freezing tolerance traits in perennial ryegrass
Is part of: Euphytica. Berlin : Springer, Vol. 204, iss. 3, 2015
Extent: p. 523-534
Date: 2015
Note: ISSN: 1573-5060 (Online)
Keywords: Association mapping;Electrolyte leakage;Proline;Winter survival
Abstract: Perennial ryegrass is an important agricultural crop, however, it is susceptible to winterkill. Freezing injury is caused primarily by ice formation. The LpIRI1 protein has the potential to inhibit ice recrystallization, thus minimize the damage. An association study was conducted using single nucleotide polymorphisms obtained through allele sequencing of the LpIRI1 gene and phenotypic data were collected using two phenotyping platforms in a perennial ryegrass association mapping population of 76 diverse genotypes. Winter survival (FWS) was evaluated under field conditions, while tiller survival (PTS) and electrolyte leakage (EL) at −8 and −12 °C were determined under controlled-environment conditions. Proline content (PC) in cold-acclimated plants was measured prior to the freezing test. Significant variation in FWS, PTS, EL and PC was observed among genotypes in our panel. EL and PTS revealed significant negative correlations at −8 °C (rs = −0.40) and −12 °C (rs = −0.49). PC, however, did not show significant correlations with any of the measured traits, while FWS was correlated (rs = −0.48) with EL at −12 °C. The LpIRI1 gene was found to be highly polymorphic with an average SNP frequency of 1 SNP per 16 bp. Association analysis revealed two non-synonymous SNPs being associated with increased EL, both located in the LpIRI1 leucine-rich repeat. The results indicate that allelic variation in the LpIRI1 gene plays an important role in the cell membrane integrity of perennial ryegrass during freezing, and can be exploited for developing more freezing tolerant cultivars
Affiliation(s): Gamtos mokslų fakultetas
Lietuvos agrarinių ir miškų mokslų centro filialas Žemdirbystės institutas
Lietuvos agrarinių ir miškų mokslų centro filialas Žemdirbystės institutas,
Vytauto Didžiojo universitetas
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications

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