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Type of publication: Straipsnis Clarivate Analytics Web of Science ar/ir Scopus / Article in Clarivate Analytics Web of Science or / and Scopus (S1)
Field of Science: Chemijos inžinerija / Chemical engineering (T005)
Author(s): Stankevič, Voitech;Saulis, Gintautas;Saulė, Rita;Bitinaitė, Aistė;Žurauskienė, Nerija;Balevičius, Saulius
Title: Theoretical analysis and experimental determination of the relationships between the parameters of the electric field pulse required to electroporate the cells
Is part of: IEEE transactions on plasma science. Santa Fe : IEEE nuclear and plasma sciences society, Vol. 41, no. 10, 2013
Extent: p. 2913-2919
Date: 2013
Keywords: Elektroporacija;Impulsinis elektrinis laukas;Pelės hepatoma;Žmogaus eritrocitai;Electroporation;Human erythrocytes;Mouse hepatoma;Pulsed electric fields
Abstract: Here, theoretical relationships between the parameters of the electric pulse, which is necessary to porate the cell by electric pulse of various shapes, have been obtained. The theoretical curves were compared with the experimental relationships. Experiments were carried out with human erythrocytes, Chinese hamster ovary and mouse hepatoma MH-22A cells. The fraction of electroporated MH-22A cells was determined from the extent of the release of intracellular potassium ions and erythrocytes-from the extent of their hemolysis after long (20-24 h) incubation in 0.63% NaCl solution at 4 oC. The dependence of the fraction of electroporated cells on the amplitude of the electric field pulse was determined for pulses with the duration from 95 ns to 2 ms. The shapes of theoretical dependencies are in agreement with experimental ones. The cell poration time depended on the intensity of the pulse: the shorter the pulse duration, the higher the electric field strength has to be. This dependence is much more pronounced for pulses < 1μs. For example, if the pulse amplitude required to electroporate 50% of human erythrocytes increased from 1.0 to 1.76 kV/cm, when the duration of a square-wave pulse was reduced from 2 ms to 20 μs, it increased from 3 to 12 kV/cm, when the pulse duration was reduced from 950 to 95 ns. The relationships between the electric field strength required for electroporation and the frequency of the applied ac field were calculated for different pulselengths. It has been obtained that although the electric field strength is constant for frequencies <10kHz but its value depends on the pulselength decreasing with increasing pulse duration. At higher frequencies, electric field strength is dependent on the frequency of the ac field
Affiliation(s): Biologijos katedra
Fizinių ir technologijos mokslų centro Puslaidininkių fizikos institutas
Gamtos mokslų fakultetas
Vytauto Didžiojo universitetas
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications

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