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Type of publication: research article
Type of publication (PDB): Straipsnis Clarivate Analytics Web of Science / Article in Clarivate Analytics Web of Science (S1)
Field of Science: Biofizika / Biophysics (N011)
Author(s): Maciulevičius, Martynas;Tamošiūnas, Mindaugas;Jurkonis, Rytis;Venslauskas, Mindaugas Saulius;Šatkauskas, Saulius
Title: Analysis of metrics for molecular sonotransfer in vitro
Is part of: Molecular pharmaceutics. Washington: ACS Publications, 2015, vol. 12, iss. 10
Extent: p. 3620-3627
Date: 2015
Keywords: Sonoporacija;Doksorubicinas;Mikroburbulų nykimo greitis;Inercinės kovitacijos dozė;Sonoporation;Metric;Doxorubicin;Microbubble sonodestruction;Inertial cavitation dose
Abstract: Ultrasound induced microbubble (MB) cavitation is used to significantly enhance cell membrane permeabilization, thereby allowing delivery of various therapeutic agents into cells. In order to monitor and quantitatively control the extent of cavitation the uniform dosimetry model is needed. In present study we have simultaneously performed quantitative evaluation of three main sonoporation factors: (1) MB concentration, (2) MB cavitation extent, and (3) doxorubicin (DOX) sonotransfer into Chinese hamster ovary cells. MB concentration measurement results and passively recorded MB cavitation signals were used for MB sonodestruction rate and spectral root-mean-square (RMS) calculations, respectively. Subsequently, time to maximum value of RMS and inertial cavitation dose (ICD) quantifications were performed for every acoustic pressure value. This comprehensive research has led not only to explanation of relation of ICD and MB sonodestruction rate but also to the development of a new sonoporation metric: the inverse of time to maximum value of RMS (1/time to maximum value of RMS). ICD and MB sonodestruction rate intercorrelation and correlation with DOX sonotransfer suggest inertial cavitation to be the key mechanism for cell sonoporation. All these metrics were successfully used for doxorubicin sonotransfer prediction (R2 > 0.9, p < 0.01) and therefore shows feasibility to be applied for future dosimetric applications for ultrasound-mediated drug and gene delivery
Affiliation(s): Biochemijos katedra
Biologijos katedra
Kauno technologijos universitetas
Vytauto Didžiojo universitetas
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications

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