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Type of publication: Straipsnis Clarivate Analytics Web of Science ar/ir Scopus / Article in Clarivate Analytics Web of Science or / and Scopus (S1)
Field of Science: Biofizika / Biophysics (N011)
Author(s): Novell, A;Escoffre, J.-M;Bouakaz, A;Lamanauskas, Nerijus;Venslauskas, Mindaugas Saulius;Šatkauskas, Saulius
Title: Bleomycin delivery into cancer cells in vitro with ultrasound and SonoVue® or BR14® microbubbles
Is part of: Journal of drug targeting. London, United Kingdm : Informa Healthcare, 2013, Vol. 21, no. 4
Extent: p. 407-414
Date: 2013
Note: Online ISSN 1029-2330
Keywords: Sonoporacija;Mikroburbulų sonodestrukcija;Bleomicinas;SonoVue;BR14 mikroburbulai;Bleomycin;BR14;Microbubble;Sonodestruction;Sonoporation;SonoVue
Abstract: Background: Cell exposure to ultrasound (US) in the presence of contrast agent microbubbles (MBs) can result in cell sonoporation that can be exploited for drug or gene delivery. Anticancer drug bleomycin (BLM), used in sonoporation, can effectively eliminate tumor cells in vitro and in vivo. Nevertheless, sonoporation mechanism is not known, thus different US parameters and MB types are used. Recently, we proposed that efficiency of cell sonoporation can be related to the efficiency of MB sonodestruction. Purpose: We analyzed human tumor cells viability in response to BLM, US and MB treatment. Methods: Human glioblastoma astrocytoma (U-87 MG) or colon cancer (HCT-116) cells were exposed to US in the presence of BLM and either SonoVue® or BR14® MBs. MB sonodestruction was evaluated according to US signal attenuation. Results: Both HCT-116 and U-87 MG cell viability following US exposure decreased up to 30%. Decrease in cell viability followed similar tendency as MB sonodestruction, which suggests direct relationship between MB sonodestruction and BLM intracellular delivery. Conclusion: Sonoporation is a feasible method to deliver BLM in to several types of human cancer cell lines. Efficiency of cell sonoporation correlated well with MB sonodestruction, providing a possibility to optimize US parameters by measuring MB sonodestruction
Affiliation(s): Biologijos katedra
Gamtos mokslų fakultetas
Vytauto Didžiojo universitetas
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications

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