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Type of publication: Tezės kituose recenzuojamuose leidiniuose / Theses in other peer-reviewed publications (T1e)
Field of Science: Biologija / Biology (N010)
Author(s): Kerienė, Ilona;Mankevičienė, Audronė;Butkutė, Bronislava;Maruška, Audrius
Title: Antifungal activity of phenolic compounds from Fagopyrum Esculentum against Fusarium culmorum
Is part of: The vital nature sign [elektroninis išteklius] : 7-th international scientific conference : abstract book. Kaunas : Vytautas Magnus university, 2013, [no. 7]
Extent: p. 62-62
Date: 2013
Abstract: The antifungal activity in vitro of phenolic extracts of buckwheat (Fagopyrum Esculentum Moench) was tested on important species of Fusarium culmorum (W.G. Smith) Sacc., which was isolated at the Department of Plant Pathology and Protection. The extracts from buckwheat grain, groats and hulls with the phenolics concentrations of 3.15 mg ml-1; 1.28 mg ml-1; 1.48 mg ml-1, respectively; as well as pure rutin and quercetin solutions at 7.52; 3.76; 1.2 mg ml-1 concentrations each, were used in the test. A small segment (0.5 cm) of the fungal growth was transferred to PDA medium, incubated at 22±2°C and the extension diameter (cm) of hyphae from centre to the sides of dishes was measured periodically for 8 days. Ethanol (96 %), without extract solutions, was used as control. The results were subjected to ANOVA using Fisher’s LSD test (p< 0.05). In all samples, significant differences in antifungal activity were detected after 96 hours incubation. Quercetin showed the strongest inhibitory effect (40%) on the mycelia growth, which lasted from the 96th hour of incubation until the end of the test (192 hours). Expansion area of fungal mycelium was 5.54±0.46 cm, compared with the control (8.60±0.05 cm). A significant inhibitory effect on F. culmorum was observed with extracts of groats and hulls. After 192 hours incubation, the area of expansion accounted for 7.39±0.49 cm and 7.19±0.37 cm, respectively. Antifungal activity of the phenols extracted from buckwheat grain was insignificant. Significant antifungal activity of rutin lasted for 144 hours. However, the differences between concentrations of quercetin and rutin in the solution did not have any influence on mycelium growth inhibition.Our research indicated no direct relationship between the higher concentration of phenols and antifungal activity, because a pure phenol compound quercetin exhibited a greater antifungal activity. [...]
Affiliation(s): Biochemijos katedra
Lietuvos agrarinių ir miškų mokslų centras
Vytauto Didžiojo universitetas
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications

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