Phylogenetic analysis of Viburnum (Viburnum) based on microsatellite markers

Abstract

Viburnum (Viburnum) - is an old plant of our country, which is characterised valuable of nutritional and medical properties. The genus Viburnum includes more than 160 species of shrubs and small trees distrubuted widely througt out Northen hemispere into the Southern hemisphere (Winkworth and Donoghue, 2004). Viburnum species in ornamental plant wich provide many ideal year-round qualities (Dean et al., 2011). Over years molecular tools have become a superior method for hybrid analysis and cultivar identification or ornamental plants (Dean et al., 2011). In Vytautas Magnus University‘s Kaunas botanical garden is a rich collection of Viburnum, more than 20 Viburnum species. With this Viburnum gene collection there were no molecular research done until now. Thirty-three microsatellite markers were tested in our researh with 18 different species of Viburnum. For phylogenetic analysis 9 selected microsatellite markers were explored Viburnum collection from VMU Kaunas Botanical Garden. After analysis the most alleles were found with primer - VDO14. The most unique alleles were found with primer -VDO12. The primer VCC_I8 was as monomorfic, primer VCC_B3 were polimorfic, seven fragments were detected. Amplitude of allele‘s size was 82 to 292 (bp). Financial support for this study was provided by the Research Council of Lithuania by project “Change of bioactive compounds in underutilized berry species depending on genotype and environment” (No.SVE-04/2011).