Please use this identifier to cite or link to this item:https://hdl.handle.net/20.500.12259/48846
Full metadata record
DC FieldValueLanguage
dc.contributor.authorŽūkienė, Rasa-
dc.contributor.authorSnitka, Valentinas-
dc.coverage.spatialNL-
dc.date.accessioned2018-10-06T22:11:33Z-
dc.date.available2018-10-06T22:11:33Z-
dc.date.issued2015-
dc.identifier.issn09277765-
dc.identifier.otherVDU02-000018251-
dc.identifier.urihttp://www.sciencedirect.com/science/article/pii/S0927776515300886#-
dc.description.abstractBovine serum albumin (BSA) and zinc oxide nanoparticles (ZnO NPs) are chosen as a model system to investigate NPs-protein corona complex formation. ZnO NPs with average size of ∼20 nm are coated with BSA using covalent and non-covalent conjugation at temperatures of 4 ◦C and 20 ◦C. The interaction mechanism between ZnO NPs and BSA is studied by using UV–vis absorption, fluorescence, synchronous fluorescence and Raman spectroscopy. Raman spectra of BSA in the presence of ZnO NPs are registered for the first time and confirm decreased -helix content, increased unstructured folding and -sheet content in BSA structure. The synchronous fluorescence spectra revealed that the hydrophobicity of the tyrosine residue is decreased and that of the tryptophan is increased. The relation of elucidated changes in BSA structure of BSA-coated ZnO NPs cytotoxicity is tested for CHO cell viability and reactive oxygen species (ROS) generation in vitro. Covalent and non-covalent binding of BSA to ZnO NPs reduces ZnO NPs cytotoxicity and ROS generation, however changes in BSA conformation makes corona less protective against ZnO NPsen
dc.description.sponsorshipBiochemijos katedra-
dc.description.sponsorshipKauno technologijos universitetas-
dc.description.sponsorshipVytauto Didžiojo universitetas-
dc.format.extentp. 316-323-
dc.language.isoen-
dc.relation.ispartofColloids and surfaces B: Biointerfaces. Elsevier B.V., 2015, Vol. 135-
dc.relation.isreferencedbyScience Citation Index Expanded (Web of Science)-
dc.relation.isreferencedbyScienceDirect-
dc.relation.isreferencedbyAcademic Search Complete (EBSCO)-
dc.relation.isreferencedbyScopus-
dc.subjectZnO nanodalelėslt
dc.subjectBSAlt
dc.subjectBaltymo struktūralt
dc.subjectRamano spektroskopijalt
dc.subjectCitotoksiškumaslt
dc.subjectROSlt
dc.subjectZnO nanoparticlesen
dc.subjectBSAen
dc.subjectProtein structureen
dc.subjectRaman spectroscopyen
dc.subjectCytotoxicityen
dc.subjectROSen
dc.subject.classificationStraipsnis Clarivate Analytics Web of Science / Article in Clarivate Analytics Web of Science (S1)-
dc.subject.otherBiochemija / Biochemistry (N004)-
dc.titleZinc oxide nanoparticle and bovine serum albumin interaction and nanoparticles influence on cytotoxicity in vitroen
dc.typeresearch article-
dc.identifier.doihttps://doi.org/10.1016/j.colsurfb.2015.07.054-
dc.identifier.isiWOS:000367410200037-
dcterms.bibliographicCitation40-
dc.date.updated2020-03-30T09:19Z-
local.object{"source": {"code": "vdu", "handle": "18251"}, "publisher": {"name": "Elsevier Science", "list": true}, "db": {"clarivate": true, "scopus": true, "list": true}, "issn": ["0927-7765"], "doi": "10.1016/j.colsurfb.2015.07.054", "code": "S1", "subject": ["N004"], "url": ["http://www.sciencedirect.com/science/article/pii/S0927776515300886#"], "country": "NL", "language": "en", "area": "N", "original": true, "pages": 8, "sheets": 0.571, "timestamp": "20200330091906.0", "account": {"year": 2015, "late": false}, "na": 2, "nip": 0, "affiliation": [{"contribution": 0.5, "aip": 2, "country": ["LT"], "rel": "aut", "org": [{"create": true, "contribution": 0.25, "name": "Vytauto Didžiojo universitetas", "id": "111950396", "level": "0", "type": "uni", "research": "1", "status": "1", "unit": {"name": "Gamtos mokslų fakultetas", "id": "01", "level": "1", "type": "fak", "research": "1", "status": "1", "unit": {"name": "Biochemijos katedra", "id": "0103", "level": "2", "type": "kat", "research": "1", "status": "1"}}}, {"create": false, "contribution": 0.25, "name": "Kauno technologijos universitetas", "id": "111950581"}], "id": "D89F30DA7ADEBE70E03604D03ED28C28", "lname": "Žūkienė", "fname": "Rasa", "status": "1", "orcid": "0000-0002-4971-0770", "name": "Žūkienė, Rasa"}, {"contribution": 0.5, "aip": 1, "country": ["LT"], "rel": "aut", "org": [{"create": false, "contribution": 0.5, "name": "Kauno technologijos universitetas", "id": "111950581"}], "id": "075FDD76B9BDCD3567C04EE9829840F2", "lname": "Snitka", "fname": "Valentinas", "status": "0", "name": "Snitka, Valentinas"}]}-
local.typeS-
item.grantfulltextopen-
item.fulltextWith Fulltext-
crisitem.author.deptBiochemijos katedra-
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications
Files in This Item:
marc.xml9.43 kBXMLView/Open

MARC21 XML metadata

Show simple item record
Export via OAI-PMH Interface in XML Formats
Export to Other Non-XML Formats


CORE Recommender

WEB OF SCIENCETM
Citations 1

49
checked on Apr 24, 2021

Page view(s)

101
checked on May 1, 2021

Download(s)

10
checked on May 1, 2021

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.