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Type of publication: research article
Type of publication (PDB): Straipsnis Clarivate Analytics Web of Science / Article in Clarivate Analytics Web of Science (S1)
Field of Science: Biochemija / Biochemistry (N004)
Author(s): Žūkienė, Rasa;Snitka, Valentinas
Title: Zinc oxide nanoparticle and bovine serum albumin interaction and nanoparticles influence on cytotoxicity in vitro
Is part of: Colloids and surfaces B: Biointerfaces. Elsevier B.V., 2015, Vol. 135
Extent: p. 316-323
Date: 2015
Keywords: ZnO nanodalelės;BSA;Baltymo struktūra;Ramano spektroskopija;Citotoksiškumas;ROS;ZnO nanoparticles;BSA;Protein structure;Raman spectroscopy;Cytotoxicity;ROS
Abstract: Bovine serum albumin (BSA) and zinc oxide nanoparticles (ZnO NPs) are chosen as a model system to investigate NPs-protein corona complex formation. ZnO NPs with average size of ∼20 nm are coated with BSA using covalent and non-covalent conjugation at temperatures of 4 ◦C and 20 ◦C. The interaction mechanism between ZnO NPs and BSA is studied by using UV–vis absorption, fluorescence, synchronous fluorescence and Raman spectroscopy. Raman spectra of BSA in the presence of ZnO NPs are registered for the first time and confirm decreased -helix content, increased unstructured folding and -sheet content in BSA structure. The synchronous fluorescence spectra revealed that the hydrophobicity of the tyrosine residue is decreased and that of the tryptophan is increased. The relation of elucidated changes in BSA structure of BSA-coated ZnO NPs cytotoxicity is tested for CHO cell viability and reactive oxygen species (ROS) generation in vitro. Covalent and non-covalent binding of BSA to ZnO NPs reduces ZnO NPs cytotoxicity and ROS generation, however changes in BSA conformation makes corona less protective against ZnO NPs
Affiliation(s): Biochemijos katedra
Kauno technologijos universitetas
Vytauto Didžiojo universitetas
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications

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