Please use this identifier to cite or link to this item:https://hdl.handle.net/20.500.12259/48490
Type of publication: conference paper
Type of publication (PDB): Tezės kituose recenzuojamuose leidiniuose / Theses in other peer-reviewed publications (T1e)
Field of Science: Biologija / Biology (N010)
Author(s): Stravinskaitė, Kristė;Zybartaitė, Lina;Kupčinskienė, Eugenija;Durka, Walter
Title: Selection of amplified fragment length polymorphism markers for investigation of genetic diversity of impatiens parviflora
Is part of: The vital nature sign [elektroninis išteklius] : 9th international scientific conference : abstract book. Kaunas : Vytautas Magnus university, 2015, [no. 9]
Extent: p. 46-46
Date: 2015
Keywords: Small balsam;Alien plants;Molecular markers;Population genetics
Abstract: Small Balsam (Impatiens parviflora) is an invasive annual usually 30-60 cm height plant with pale yellow flowers, which has red spots on the inside. Impatiens parviflora is a native of Central Asia, this species is widely distributed in eastern, central and northern European countries and it is one of the most invasive species in the continent [1]. The objective of this study was to select amplified fragment length polymorphism (AFLP) markers for investigation of genetic diversity of Impatiens parviflora. Four individuals were chosen randomly from European populations and were used to screen AFLP primer combinations. Fragment analysis was performed on an ABI 3130 genetic analyser using GeneScan LIZ 500 as internal size standard. Peaks were scored manually in the range of 50-500 bp using GeneMapper version 3.7. In total 32 primer combinations were screened. Eight fluorescent labelled primers (FAM-AAC/CTC, VIC-ACG/CAC, NED-ACC/CAC, PET-AGG/CAC and FAM-AAC/CTG, VIC-ACG/CAT, NED-AAG/CAG, PET-AGC/CAC) were selected according to generated peak height and their distance from each other. We eliminated primers, which composed low peaks or generated peaks were too close to each other or too distant from each other, as it was described by Kloss et al., 2011 [2]. Results revealed that chosen AFLP markers are valuable for estimation of genetic diversity of Impatiens parviflora populations
Internet: https://hdl.handle.net/20.500.12259/48490
Affiliation(s): Biologijos katedra
Gamtos mokslų fakultetas
Vytauto Didžiojo universitetas
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications

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