Please use this identifier to cite or link to this item:https://hdl.handle.net/20.500.12259/48427
Type of publication: Straipsnis Clarivate Analytics Web of Science ar/ir Scopus / Article in Clarivate Analytics Web of Science or / and Scopus (S1)
Field of Science: Biologija / Biology (N010)
Author(s): Sungailaitė, Sandra;Ruzgys, Paulius;Šatkauskienė, Ingrida;Čepurnienė, Karolina;Šatkauskas, Saulius
Title: The dependence of efficiency of transmembrane molecular transfer using electroporation on medium viscosity
Is part of: Journal of gene medicine. New-York, USA : Wiley-Blackwell, 2015, Vol. 17, iss. 3-5
Extent: p. 80-86
Date: 2015
Note: Impact Factor: 1.953. eISSN 1521-2254
Keywords: Bleomycin;DNA electrotransfer;Drug delivery;Electroporation;Transfection;Viscosity
Abstract: In the present study, we aimed to evaluate the efficiency of drug and gene electrotransfer into cells in vitro depending on medium viscosity. Methods Experiments were performed using Chinese hamster ovary cells. Efficiency of molecular electrotransfer depending of medium viscosity was evaluated using two different electroporation conditions: a high-voltage (HV) pulse and a combination of a high-voltage pulse and a low-voltage pulse (HV + LV). To evaluate the efficiency of molecular electrotransfer, anticancer drug bleomycin and two different plasmids coding for green fluorescent protein and luciferase were used. Results We found that a slight increase in medium viscosity from 1.3–1.4 mPa·s significantly decreased the transfection efficiency, both in terms of transfected cells and total protein production, which was abolished completely with an increase in medium viscosity to 6.1 mPa·s. Notably, at this medium viscosity, electrotransfer of the small anticancer drug was still efficient. Using HV and HV + LV pulse combinations, we showed that a decrease of DNA electrotransfer, especially at lower medium viscosities, can be compensated for by the LV pulse to some extent. On the other hand, the addition of the LV pulse after the HV pulse did not have any positive effect on the efficiency of bleomycin electrotransfer. Conclusions These findings demonstrate that transfection is very susceptible to medium viscosity and highlights the importance of the electrophoretic component in experiments when a considerable transfection level is needed
Internet: http://onlinelibrary.wiley.com/doi/10.1002/jgm.2825/abstract
http://onlinelibrary.wiley.com/doi/10.1002/jgm.2825/abstract
Affiliation(s): Biologijos katedra
Gamtos mokslų fakultetas
Vytauto Didžiojo universitetas
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications

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