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Type of publication: research article
Type of publication (PDB): Straipsnis Clarivate Analytics Web of Science / Article in Clarivate Analytics Web of Science (S1)
Field of Science: Biologija / Biology (N010)
Author(s): Marcinkevičiūtė, Aušra;Mildažienė, Vida;Crumm, Sara;Demin, Oleg;Hoek, Jan B;Kholodenko, Boris
Title: Kinetics and control of oxidative phosphorylation in rat liver mitochondria after chronic ethanol feeding
Is part of: Biochemical journal. London : Portland press, 2000, vol. 349, iss. 2
Extent: p. 519-526
Date: 2000
Keywords: Biochemija;Control coefficient;Kinetic response;Top-down control analysis
Abstract: Changes in the kinetics and regulation of oxidative phosphorylation were characterized in isolated rat liver mitochondria after 2 months of ethanol consumption. Mitochondrial energy metabolism was conceptually divided into three groups of reactions, either producing protonmotive force (Delta p) (the respiratory subsystem) or consuming it (the phosphorylation subsystem and the proton leak), Manifestation of ethanol-induced mitochondrial malfunctioning of the respiratory subsystem was observed with various substrates; the respiration rate in State 3 was inhibited by 27 +/- 4% with succinate plus amytal, by 20 +/- 4% with glutamate pins malate, and by 17+/-2% with N,N,N',N'-tetramethyl-p-phenylencdiamine/ascorbate. The inhibition of the respiratory activity correlated with the lower activities of cytochrome c oxidase, the bc(1) complex, and the ATP synthase in mitochondria of ethanol-fed rats. The block of reactions consuming the Delta p to produce ATP (the phosphorylating subsystem) was suppressed after 2 months of ethanol feeding, whereas the mitochondrial proton leak was not affected. The contributions of Delta p supply (the respiratory subsystem) and Delta p demand (the phosphorylation and the proton leak) to the control of the respiratory flux were quantified as the control coefficients of these subsystems. In State 3, the distribution of control exerted by different reaction blocks over respiratory flux was not significantly affected by ethanol diet, despite the marked changes in the kinetics of individual functional units of mitochondrial oxidative phosphorylation. This suggests the operation of compensatory mechanisms, when control redistributes among the different components within the same subsystem
Affiliation(s): Gamtos mokslų fakultetas
Kauno medicinos universiteto Biomedicininių tyrimų institutas
Vytauto Didžiojo universitetas
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications

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