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Type of publication: Straipsnis Clarivate Analytics Web of Science ar/ir Scopus / Article in Clarivate Analytics Web of Science or / and Scopus (S1)
Field of Science: Biologija / Biology (N010)
Author(s): Mildažienė, Vida;Banienė, Rasa;Naučienė, Zita;Marcinkevičiūtė, Aušra;Morkūnienė, Ramunė;Borutaitė, Vilmantė;Kholodenko, Boris;Brown, Guy C
Title: Ca2+ stimulates both the respiratory and phosphorylation subsystems in rat heart mitochondria
Is part of: Biochemical journal. London : Portland press, 1996, vol. 320, part. 1
Extent: p. 329-334
Date: 1996
Note: Bibliogr.: 24 pavad
Keywords: ATP synthesis;Calcium-Ions;Intramitochondrial Metabolism;Oxidative-Phosphorylation;Mammalian Mitochondria;Muscle-Cells;Dehydrogenase;Invivo;Complex;Ca2+
Abstract: Stimulation of mitochondrial respiration by physiological concentrations of Ca2+ was studied to determine which components of oxidative phosphorylation are affected by Ca2+. The kinetic dependence of the respiratory chain, phosphorylation subsystem and proton leak on the mitochondrial membrane potential in isolated rat heart mitochondria respiring on 2-oxoglutarate or succinate was measured at two different concentrations of external free Ca2+. The results show that proton leak is not directly affected by Ca2+, but that both the respiratory and phosphorylation systems can be directly stimulated by Ca2+ depending on conditions. Although Ca2+ directly stimulates the phosphorylation system, this has relatively little effect on respiration rate with 2-oxoglutarate in States 3 and 4 because the subsystem has little control over respiration. However, in intermediate states, the phosphorylation system has greater control and Ca2+ stimulation of this system contributes substantially to the stimulation of respiration and phosphorylation. In the case of succinate oxidation neither the respiratory subsystem nor the phosphorylation system is stimulated by Ca2+
Affiliation(s): Kauno medicinos universiteto Biomedicininių tyrimų institutas
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications

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