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Type of publication: research article
Type of publication (PDB): Straipsnis Clarivate Analytics Web of Science / Article in Clarivate Analytics Web of Science (S1)
Field of Science: Biochemija / Biochemistry (N004)
Author(s): Mildažienė, Vida;Banienė, Rasa;Marcinkevičiūtė, Aušra;Naučienė, Zita;Kalvėnas, Alvydas;Zimkus, Aurelijus
Title: Tetraphenylphosphonium inhibits oxidation of physiological substrates in heart mitochondria
Is part of: Molecular and cellular biochemistry. Dordrecht : Kluwer Academic, 1997, vol. 174, no. 1-2
Extent: p. 67-70
Date: 1997
Keywords: Heart mitochondria;Oxidative phosphorylation;Membrane potential;Tetraphenylphosphonium
Abstract: We show that tetraphenylphosphonium inhibits oxidation of palmitoylcarnitine, pyruvate, malate, 2-oxoglutarate and glutamate in heart mitochondria in the range of concentration (1-5 mu M) commonly used for the determination of mitochondrial membrane potential. The inhibition of 2-oxoglutarate (but not other substrate) oxidation by tetraphenylphosphonium is dependent on the concentration of 2-oxoglutarate and on extramitochondrial free calcium, and the kinetic plots are consistent with a mixed type of inhibition. Our results indicate that tetraphenylphosphonium interacts with enzymes, specifically involved in the oxidation of 2-oxoglutarate, most possibly, 2-oxoglutarate dehydrogenase
Affiliation(s): Kauno medicinos universiteto Biomedicininių tyrimų institutas
Lietuvos kūno kultūros akademija
Vilniaus universitetas
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications

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