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Type of publication: research article
Type of publication (PDB): Straipsnis Clarivate Analytics Web of Science / Article in Clarivate Analytics Web of Science (S1)
Field of Science: Biologija / Biology (N010)
Author(s): Vinga, Ines;Dröge, Anja;Stiege, Asita C;Lurz, Rudi;Santos, Mario A;Daugelavičius, Rimantas;Tavares, Paulo
Title: The minor capsid protein gp7 of bacteriophage SPP1 is required for efficient infection of Bacillus subtilis
Is part of: Molecular microbiology. Oxford : Blackwell publishing Ltd, 2006, vol. 61, no. 6
Extent: p. 1609-1621
Date: 2006
Abstract: Gp7 is a minor capsid protein of the Bacillus subtilis bacteriophage SPP1. Homologous proteins are found in numerous phages but their function remained unknown. Deletion of gene 7 from the SPP1 genome yielded a mutant phage (SPP1del7) with reduced burst-size. SPP1del7 infections led to normal assembly of virus particles whose morphology, DNA and protein composition was undistinguishable from wild-type virions. However, only 25% of the viral particles that lack gp7 were infectious. SPP1del7 particles caused a reduced depolarization of the B. subtilis membrane in infection assays suggesting a defect in virus genome traffic to the host cell. A higher number of SPP1del7 DNA ejection events led to abortive release of DNA to the culture medium when compared with wild-type infections. DNA ejection in vitro showed that no detectable gp7 is co-ejected with the SPP1 genome and that its presence in the virion correlated with anchoring of released DNA to the phage particle. The release of DNA from wild-type phages was slower than that from SPP1del7 suggesting that gp7 controls DNA exit from the virion. This feature is proposed to play a central role in supporting correct routing of the phage genome from the virion to the cell cytoplasm
Affiliation(s): Vilniaus universitetas
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications

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