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Type of publication: research article
Type of publication (PDB): Straipsnis recenzuojamoje Lietuvos tarptautinės konferencijos medžiagoje / Article in peer-reviewed Lithuanian international conference proceedings (P1e)
Field of Science: Biofizika / Biophysics (N011)
Author(s): Venslauskas, Mindaugas Saulius
Title: Mažų, bioaktyvių molekulių elektropernašos į ląsteles, taikant impulsinius elektrinius laukus, optimizacija
Other Title: Optimisation of electrotransfer of small bioactive molecules into cells in vitro by using pulse electric fields
Is part of: Biomedicininė inžinerija : tarptautinės konferencijos pranešimų medžiaga = Biomedical Engineering : Proceedings of International Conference (2005 m. spalio 27-28 d., Kaunas). Kaunas : Technologija, 2005
Extent: p. 132-139
Date: 2005
Keywords: Ląstelės;Bioaktyvios molekulės;Elektriniai laukai;Cells;Bioactive molecules;Electric fields
ISBN: 995509950X
Abstract: Theoretical background and experimental measurements of estimation probability electroporation of needed amount of the cells in suspension as a function of electric field parameters are presented. Three steps in process of high electric field pulse generators-electroporators construction, manufacturing and testing carried out in our laboratory are discussed. Aiming to measure theoretically predicted probability of electroporation cells in vitro the erythrocytes and hepatoma MH22 line cells were used. The electroporation efficiency was estimated by two different methods: (i) measuring efflux of intracellular K+ by using mini selective and reference electrodes (ii) counting the cells killed by anticancer drug bleomycin. It was shown that on the basis measured electroporation probability versus electric field parameters, the optimal set of pulse strengthduration combinations allowing transfer of chosen small molecules into defined amount of cells could be predicted and the size of electropores could be estimated
Affiliation(s): Biologijos katedra
Vytauto Didžiojo universitetas
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications

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